Abstract:
This research aimed to using Spent coffee grounds (SCG) and Rain tree leaves (RTL) as a mushroom substrate as like as rubber sawdust (SW). The result showed mixing SCG at ratio of 30% (w/w) could increase a protein content, 35.08±0.59% and weight of product, 80.33±2.15 g, but increasing cultivations time to about two times, 64 days, compared with using SW substrate alone. The fruiting bodies from 30% (w/w) mixing SCGs substrate had a caffeine at 1.70 mg/L. Using RTL at 30% (w/w) ratio could also had same equivalent product in term of protein, weight, and cultivation time as only using SW as substrate alone, there are 31.64±0.76%, 55.90±3.18 g and around 37 days respectively. The phenolic compound (TPC) was found in slightly difference in each mushroom substrate. Next, the spent mushroom substrate with component as SW, SCG and RTL could be used as biomass but should be reduced the moisture.