Abstract:
This study aimed to comprehensively evaluate the Quality Assessment and
Bioactive Potential Evaluation of Six Medicinal Plants from the Jantaleela Traditional
Formulation: Tarenna hoaensis Pit. , Dracaena loureiroi Gagnep. , Pogostemon cablin
( Blanco) Benth. , Phyllanthus emblica L. , Terminalia bellirica ( Gaertn. ) Roxb. , and
Terminalia chebula Retz. , which serve as essential raw materials in Thai traditional
medicine formulations. Quality assessment revealed optimal moisture content (6. 26
7.73 g/100 g) and ash content within acceptable standards (1.6412.56 g/100 g), indicating
proper post-harvest processing and storage suitability. Microbiological safety screening
detected no contamination of pathogenic organisms including Salmonella spp. ,
Staphylococcus aureus, and Clostridium spp. in all samples, fully complying with World
Health Organization ( WHO) guidelines for herbal medicines. Antibacterial activity
assessment via Disk Diffusion method against Escherichia coli and S. aureus
demonstrated moderate inhibition zones ( 8. 2 11. 87 mm) , attributed to phenolic
compounds and tannins present in these herbs. Antioxidant capacity evaluated through
Total Phenolic Content, Ferric Reducing Antioxidant Power (FRAP), and 2,2-diphenyl-1-
picrylhydrazyl (DPPH) assays revealed remarkable potency in P. emblica and T. chebula
(882.6221,524.36 mg Trolox/g for Total Phenolic Content and 3,754.34 mg Trolox/g for
FRAP), while D. loureiroi and T. bellirica excelled in DPPH (1,116.41 mg Trolox/g) and
Total Phenolic Content (18,157.62 mg Trolox/g), respectively. Comprehensive screening
for 40 pesticide residues across three major groups ( Carbamate, Organochlorine,
Organophosphate, and Pyrethroid) and four heavy metals (Arsenic, Lead and Cadmium)
confirmed absence of contamination exceeding WHO safety thresholds. These findings
collectively affirm that all six herbs exhibit exceptional safety profiles, freedom from
hazardous contaminants, and possess significant antioxidant and antimicrobial
bioactivities, rendering them highly suitable for pharmaceutical applications in Thai
traditional medicine formulations such as Jantaleela, supporting holistic therapeutic
approaches and health product development.