Antioxidant activity and total phenolic content of various solvent extracts of Gymnanthemum extensum leaf

Name: Sukanya Khiaosa-at

Organization : Payap University. Faculty of Pharmacy

Email : sukanya_k40@hotmail.com

Name: Chanokporn Choochat

Organization : Payap University. Office of General Education

Name: Pisamai Kijkuokool

Organization : Payap University. Office of General Education

Name: Surapol Natakankitkul

Organization : Payap University. Graduate School

keyword: Gymnanthemum extensum

LCSH: Medicinal plants

LCSH: Antioxidants -- Analysis

LCSH: Phenols -- Analysis

LCSH: Plant extracts

Abstract: Gymnanthemum extensum has been used as traditional medicine for reducing blood sugar, blood pressure, and blood lipid. There are few reports in the literature mentioning the antioxidant activity and total phenolic content of G. extensum leaf. The present study aimed to investigate the antioxidant activity and total phenolic content of various solvent extracts of G. extensum leaf. The leaves were extracted with different solvents, including n-hexane, ethyl acetate, methanol, and hot water. The yields of extraction decreased in the following order: methanol > ethyl acetate > water > n-hexane. All extracts were evaluated for antioxidant activity using the DPPH and ABTS radical scavenging assays. The total phenolic content was determined by Folin-Ciocalteu colorimetric assay. The results indicated that the aqueous extract showed the highest antioxidant activity by DPPH assay with the IC50 value of 0.099±0.001 mg/mL, followed by the methanol (0.308±0.005 mg/mL), ethyl acetate (1.793±0.031 mg/mL) and n-hexane (2.531±0.064 mg/mL) extracts, respectively. Also, the aqueous extract had the highest antioxidant activity against ABTS radical with the IC50 value of 0.116±0.003 mg/mL, followed by the methanol (0.333±0.003 mg/mL), ethyl acetate (0.452±0.013 mg/mL) and n-hexane (0.653±0.010 mg/mL) extracts, respectively. The aqueous extract exhibited the highest total phenolic content which contained 138.99±1.75 mgGAE/g, followed by the methanol (47.02±1.79 mgGAE/g), ethyl acetate (36.83±1.09 mgGAE/g) and n-hexane (20.45±0.53 mgGAE/g) extracts, respectively. These results indicate that water is the best solvent for extracting phenolic compounds from G. extensum leaf for antioxidant activity. So, the aqueous extract of G. extensum leaf may have health benefits as a potential source for antioxidant agents in food and medicine applications.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2025

Modified: 2025-10-28

Issued: 2025-10-28

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BibliograpyCitation : In The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana and Suranaree University of Technology. Institute of Science. School of Chemistry. Pure and Applied Chemistry International Conference 2025 (PACCON 2025) (pp.536-539). Bangkok : The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana in association with the School of Chemistry, Institute of Science, Suranaree University of Technology, 2025

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