Antioxidant activity of protein hydrolysates from paddy straw mushroom (Volvariella volvacea)

Name: Parita Nutwuttisak

Organization : Phranakhon Si Ayutthaya Rajabhat University. Faculty of Science and Technology

Name: Chanthakan Nuchsuk

Organization : Phranakhon Si Ayutthaya Rajabhat University. Faculty of Science and Technology

Email : pui.chanthakan@gmail.com

keyword: Alcalase

LCSH: Volvariella volvacea

LCSH: Mushrooms -- Analysis

LCSH: Protein hydrolysates

Abstract: This study aimed to produce protein hydrolysates from crude protein extracts of paddy straw mushroom (Volvariella volvacea) at three developmental stages—button, egg, and elongation—using the commercial enzyme Alcalase and to assess their antioxidant properties. Crude protein extracts were prepared in phosphate-buffered saline (pH 7.4), with the button stage having the highest protein concentration (8.86±0.04 mg/mL). Hydrolysis was performed at 60 °C with enzyme concentrations of 0.125–0.50% (v/v) for up to 60 min. The highest degree of hydrolysis (36.48±0.26%) was achieved at 0.5% enzyme for 30 min. Antioxidant activity was evaluated using DPPH and FRAP assays. Crude extracts (1 mg/mL) showed DPPH scavenging of 35.28±0.35% (button), 38.34±0.53% (egg), and 29.86±0.81% (elongation), while hydrolysates exhibited higher activity: 77.51±0.20%, 86.83±0.40%, and 63.31±0.88%, respectively. FRAP values were also higher for hydrolysates, with the egg stage showing the strongest activity (8.85±0.05 μmoL Fe²⁺/g). These findings suggest that protein hydrolysate from V. volvacea, particularly from the egg stage, displayed highest antioxidant activity in both assay.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2025

Modified: 2025-10-28

Issued: 2025-10-28

บทความ/Article

application/pdf

BibliograpyCitation : In The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana and Suranaree University of Technology. Institute of Science. School of Chemistry. Pure and Applied Chemistry International Conference 2025 (PACCON 2025) (pp.526-531). Bangkok : The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana in association with the School of Chemistry, Institute of Science, Suranaree University of Technology, 2025

eng

©copyrights King Mongkut's University of Technology North Bangkok

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	PACCON 2025pp.526-531.pdf	1.17 MB

ใช้เวลา

0.021597 วินาที