Development of lateral flow device for detection of polystyrene nanoplastics

Name: Rawisara Wadeesirisak

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Kanjana Kunpatee

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Sirowan Ruantip

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Sudkate Chaiyo

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Email : sudkate.c@chula.ac.th

keyword: Gold nanoparticles

LCSH: Nanoplastics -- Detection

; Lateral flow immunoassay

LCSH: Polystyrene -- Analysis

Abstract: Nanoplastics are tiny particles of synthetic polymers or plastics smaller than 100 nanometers. The most common nanoplastics, such as polystyrene (PS), have attracted much attention from various plastics sectors. Conventional methods of PS nanoplastics detection often require skilled operators, are timeconsuming, and have high equipment costs. Therefore, a lateral flow device for nanoplastics detection was proposed because it is easy to use, low cost, portable, does not require expensive instruments, and enables efficient field analysis. This device applied a colorimetric method with lateral flow using mercaptoundecanoic acid (MUA) and antibody modified on gold nanoparticles (AuNPs) surface to detect PS nanoplastics. The detection mechanism is based on hydrophobic interaction between MUA-AuNP and PS nanoplastics to suppress the pH-induced aggregation of MUA functionalized on AuNPs. In the absence of PS, free MUAAuNPs aggregate with HCl, so they cannot flow to the T-line and, therefore, do not appear as a color band. In the presence of PS, it results in anti-aggregation of MUA-AuNPs, so it can flow to the T-line and appear as a color band. Under optimized conditions, the presented method can rapidly visualize PS nanoplastics with a 100 ng/mL detection limit. This device successfully detected PS nanoplastics in boiled water and hot tea samples. Consequently, it can be shown that this device can detect PS nanoplastics with attractive, low-cost, portable, and can serve as an analytical tool for monitoring PS nanoplastics contamination

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2025

Modified: 2025-10-22

Issued: 2025-10-22

บทความ/Article

application/pdf

BibliograpyCitation : In The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana and Suranaree University of Technology. Institute of Science. School of Chemistry. Pure and Applied Chemistry International Conference 2025 (PACCON 2025) (pp.103-109). Bangkok : The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana in association with the School of Chemistry, Institute of Science, Suranaree University of Technology, 2025

eng

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