Development of a microfluidic paper-based electrochemical immunosensor for detection of aflatoxin M1

Name: Parichat Saritcharatkun

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Walaiporn Veyrudit

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Kittinan Komolpis

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Nanthika Khongchareonporn

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Sudkate Chaiyo

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Email : sudkate.c@chula.ac.th

keyword: Aflatoxin M1

LCSH: Aflatoxins -- Detection

; Electrochemical detection

LCSH: Biosensors -- Design and construction

LCSH: Graphene

Abstract: Aflatoxins are a class of secondary metabolites produced by Aspergillus species and are commonly found in agricultural products such as grains and crops. Aflatoxin B1 (AFB1) is a major aflatoxin, and its metabolite, Aflatoxin M1 (AFM1), poses significant health risks. To prevent severe milk contamination, the Food and Drug Administration of Thailand has set the maximum allowable AFM1 concentration at 0.5 μg/L. Conventional methods for AFM1 detection, such as high-performance liquid chromatography (HPLC), are effective but expensive and require specialized expertise. In this study, we developed a label-free biosensor on a paper-based platform to provide a simple and affordable detection method. This approach introduced electrochemical detection using graphene screen-printed electrodes integrated into a microfluidic paper-based analytical device (μPAD) for AFM1 detection in milk. The μPAD consisted of a redox zone, sample zone, detection zone, and absorbent zone, all interconnected by microchannels. The redox zone served as a reservoir for ferric/ferrocyanide redox species, while the detection zone housed graphene electrodes immobilized with anti-AFM1 antibodies. The developed μPAD sensor demonstrated a detection limit of 0.5 μg/L for AFM1. Using simple in-house fabrication, this electrochemical μPAD immunosensor is inexpensive, portable, and suitable for on-site detection of AFM1 in milk

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2025

Modified: 2025-10-21

Issued: 2025-10-21

บทความ/Article

application/pdf

BibliograpyCitation : In The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana and Suranaree University of Technology. Institute of Science. School of Chemistry. Pure and Applied Chemistry International Conference 2025 (PACCON 2025) (pp.7-12). Bangkok : The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana in association with the School of Chemistry, Institute of Science, Suranaree University of Technology, 2025

eng

©copyrights King Mongkut's University of Technology North Bangkok

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	PACCON 2025pp.7-12.pdf	821.19 KB

ใช้เวลา

0.036376 วินาที