Abstract:
This study aimed to analyze the chemical composition, specifically the protein content and 10-hydroxy-2-decenoic acid (10-HDA), in royal jelly derived from wildflower pollen in Mae Sai District, Chiang Rai Province, and to compare the results with the Thai quality standard. Three samples were collected in August 2024 from Apis mellifera hives maintained on honey farms certified under Good Agricultural Practices (GAP) for beekeeping. Protein content was determined using the Kjeldahl method, and 10-HDA content was analyzed using High Performance Liquid Chromatography (HPLC). The results showed an average protein content of 13.92 ± 0.57% and an average 10-HDA content of 1.97 ± 0.03%, both exceeding the minimum thresholds of the Thai quality standard (≥11% for protein and ≥1.5% for 10-HDA). However, statistical analysis using the Wilcoxon Signed-Rank Test revealed no significant difference (p = 0.055) between the sample medians and the standard values. In conclusion, the royal jelly from wildflower pollen examined in this study met the Thai quality standard, which is important for the development and value addition of royal jelly in the countrys health and beauty industries.