Studies of biological activities and chemical profiles of Hericium erinaceus mushroom cultivated by novel medium formula

Name: Sari Darmasiwi

LCSH: Kasetsart University -- Dissertations. Ph.D. (Microbiology) 2021

Classification :.LCCS: QK629.H8

LCSH: Kasetsart University. -- Department of Microbiology -- Dissertations

LCSH: Hericium erinaceus

LCSH: Edible mushrooms

LCSH: Antioxidants

LCSH: Antineoplastic agents

Abstract: Hericium erinaceus is an edible mushroom with recognized medicinal value that naturally lives in subtropical regions. The use of solid cultures in the exsitu cultivation of mushrooms has been reported to improve the nutritive value, bioactive compounds, and certain biological activities of fungal products. An artificial cultivation of H. erinaceus using different medium formula of jasmine rice, white jasmine rice (R1), and a mixture of red and white jasmine rice (R2), was applied to study the biological activities and chemical profiles of the cultivated products. The results showed that H. erinaceus was successfully cultivated on different jasmine rice media. Mycelia growth was positively correlated with the pH of the media. The rice fermented mycelia (MR1 and MR2) had higher protein levels compared to the unfermented rice controls (R1 and R2). Basidiomata products (BR1 and BR2) exhibited higher protein levels compared to commercial basidiome (BS). The rice fermented mycelia contained alkaloids, terpenoids, and saponins, while cultivated basidiomata contained terpenoids and saponins. The preliminary screening of biological activities of the cultivated products on different solvents showed that ethanol extract had better antioxidant and antimicrobial activities compared to water extract. Among the other samples, the ethanol extract of MR2 had the highest antimicrobial activity, particularly against pathogens such as Salmonella Typhimurium and Proteus mirabilis (MIC=12.5-25 mg/ml). BS had higher antibiofilm activity compared to other products. Furthermore, the ethanol extract of MR2 presented inhibition against cervical cancer cell line (HeLa) with IC50=1447.8 µg/ml, 526.7 µg/ml, and 481.9 µg/ml at 24, 48, and 72 h, respectively. The HPLC-MS analysis showed that MR2 comprised a significant amount of phenolic compounds such as p-coumaric acid (43.33 µg/ml), p-hydroxybenzoic acid (41.69 µg/ml), ferulic acid (21.90 µg/ml), gallic acid (17.55 µg/ml), and catechin derivatives (18.07 µg/ml). The fractionation of MR2 gained three different fractions, with the lipophilic fraction (LF2) presenting the highest antimicrobial activity against Proteus mirabilis (MIC=250µg/ml). The GC-MS analysis of fractions contained fatty acid derivatives, mainly nonanoic acid, 2-dodecen-1-yl(-)succinic anhydride, linoleic acid ethyl ester, 3,4-dimethylbenzoic acid, and lauryl acetate as antimicrobial compounds. Nevertheless, the LF2 fractions had low anticancer activities (IC50>100µg/ml). This study suggests that MR2 of H. erinaceus could be used as a potential antimicrobial agent.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Ingorn Kimkong

Role: Thesis Advisor

Name: Yaovapa Aramsirirujiwet

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-27

Issued: 2025-07-27

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/sari-dar-all.pdf

CallNumber: QK629.H8 .S27

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Microbiology

Grantor: Kasetsart University

©copyrights Kasetsart University

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