Energy metabolism monitoring by proteomic and metabolomic analyses in Rats administrated with Crocodile oil (Crocodylus siamensis) supplement

Name: Kongphop Parungakul

LCSH: Kasetsart University -- Dissertations. (Zoology) 2022

Classification :.LCCS: QP176

LCSH: Kasetsart University. -- Department of Zoology -- Dissertations

LCSH: Energy metabolism

LCSH: Fatty acids

LCSH: Proteomics

LCSH: Crocodile oil

Abstract: Consumption of fatty acids (FA) can alter whole energy metabolism and hepatic mitochondrial function. Crocodile oil (CO) is rich in mono-and polyunsaturated fatty acids, which have natural anti-inflammatory and healing properties. To determine the alteration of liver energy metabolism, we investigated the effect of CO on mitochondrial function for energy homeostasis. Twenty-one male Sprague-Dawley rats were randomly divided into three groups. Group 1 rats were given sterile water (RO), Group 2 rats were given CO (3% v/w), and Group 3 rats were given palm oil (PO) (3% v/w). The rats were given sterile water, CO, and PO orally for 7 weeks. Furthermore, to determine the alteration of energy metabolic signature in serum, we investigated different doses of CO consumption on the expression of proteins involved in energy metabolism in rats. Twenty-one male Sprague-Dawley rats were divided into three groups and administered sterile water (N) or different doses of CO (1% or 3% [v/w] CO) orally once daily for 8 weeks. Body weight, food intake, energy intake, blood lipid profiles, and energy-related metabolites were determined. Mitochondrial architecture, and hydrolase domain containing 3 (HDHD3) protein expression in liver mitochondria were studied. The serum proteome was analyzed using shotgun proteomics, and the functions of candidate proteins were classified using PANTHER software. The results showed that there are no differences in body weight, liver weight, liver index, energy intake, and lipid profiles. The CO group consumed significantly less food than the control group. The CO group also had significantly higher levels of liver oxaloacetate and malate than the PO group. CO treatment significantly ameliorated hepatic steatosis, as evidenced by a greater decrease in the total surface area of lipid particles than PO treatment. CO administration preserved hepatic mitochondrial morphology by upregulating the energetic maintenance protein HDHD3. Furthermore, chemical-protein interactions revealed that HDHD3 was linked to the energy homeostatic pathway. In the context of different doses effect of CO, both 1% and 3%CO-treated groups showed significantly decreased serum triglyceride (TG) levels. Remarkably, 1C% CO exhibited significantly lower TG levels in post-treatment than in pre-treatment. The serum oxaloacetate level was also significantly higher in CO groups than in the N group. The proteomic analysis revealed that more unique proteins are involved in cellular metabolic activity in both CO-treated groups than in the N group. Clustering analysis of 295 shared differentially expressed proteins in both CO-treatment groups showed that upregulated hyper-expressed protein clusters were associated with catalytic activity and molecular activity on the same levels. This research concluded that CO may preserve hepatic mitochondrial architecture, increase energy metabolic activity, and improve lipid profiles.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Wirasak Fungfuang

Role: Thesis Advisor

Name: Phitsanu Tulayakul

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-27

Issued: 2025-07-27

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/kongphop-par-all.pdf

CallNumber: QP176 .K66

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Zoology

Grantor: Kasetsart University

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