Effects of Carbon nanoparticles on biological membrane

Name: Nililla Nisoh

LCSH: Kasetsart University -- Dissertations. Ph.D. (Physics) 2021

Classification :.LCCS: QC176.8.N35

LCSH: Kasetsart University. -- Department of Physics -- Dissertations

LCSH: Nanoparticles

LCSH: Carbon

LCSH: Membranes (Biology)

LCSH: Molecular dynamics

Abstract: Understanding the interactions between carbon nanoparticles (CNPs) and biological membranes is critical in applications of CNPs in biomedical and toxicology. Several experimental and computational studies have shown that fullerene aggregates are harmful to biological membranes. Importantly, the mechanisms of membrane disruption remain poorly understood, and fullerene aggregation (or lack of it) in bilayers at high concentrations remains a crucial open question. Due to the complex mixtures of the systems, most studies have focused on the interactions of CNPs and single component bilayers. In this work, coarse-grained molecular dynamics (CG-MD) simulations were performed to investigate the behavior of fullerene in cell membranes as a function of fullerene concentration. We used two group of model cell membranes consisting of single lipid component bilayers (vary lipid saturations and chain lengths) and multiple lipid components in the plasma membranes. Our results reveal that how fullerenes behave and where the fullerenes are located inside a lipid bilayer depends on the degree of lipid saturation. In saturated and monounsaturated bilayers, fullerenes aggregated and formed icosahedral structures. In polyunsaturated lipid bilayers, qualitatively different behavior was observed with the fullerenes forming a connected percolation network at midplane. In the case of plasma membranes, fullerenes prefer to be located in the region of the highly unsaturated lipids which are enriched in the inner leaflet of the plasma membrane. This causes fullerene aggregation even at low concentrations. When increasing fullerene concentration, the fullerene clusters grow and budding may emerge at the inner leaflet of the plasma membrane. Moreover, the mechanisms of how fullerenes perturb multicomponent cell membrane and how they directly enter the cell are proposed. These insights can help to determine fullerene toxicity in living cells.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Jirasak Wong-Ekkabut

Role: Thesis Advisor

Created: 2021

Modified: 2025-07-26

Issued: 2025-07-26

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/nililla-nis-all.pdf

CallNumber: QC176.8.N35 .N55

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Physics

Grantor: Kasetsart University

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