Proteomic analysis of equine melanocytic neoplasm in the grey coat color

Name: Parichart Tesena

LCSH: Kasetsart University -- Dissertations. Ph.D. (Animal Health and Biomedical Sciences) 2022

Classification :.LCCS: SF951

LCSH: Kasetsart University. -- Faculty of Veterinary Medicine -- Dissertations

LCSH: Horses -- Diseases

LCSH: Melanoma

LCSH: Proteomics

Abstract: Equine melanocytic neoplasm (EMN) is cutaneous neoplasm and frequently detects in aged grey horses. This study aimed to examine the profiles proteins expression of EMN in serum and feces samples. This study reveals the potential proteins in less to non-invasive approach on EMN using proteomics from serum and feces samples. All of the samples were collected from twenty-five grey horses which were assigned into three groups containing normal (free of EMN; n=10), mild EMN (n=6) and severe EMN (n=9) in pony, Lusitano and Thoroughbred breeds. The grey horses were collected the blood and feces. Subsequently, the Liquid Chromatography-Tandem Mass spectrometry were used for analysis and identification of the protein. According the differentially expressed proteins DEPs, the significant proteins were categorized involved in metabolism and non-metabolism functions according to KEGG database. The serum results showed that BRCA1, phosphorylase b kinase regulatory subunit: PHKA1, tyrosineprotein kinase receptor: ALK and rho-associated protein kinase: ROCK1 correlating to EMN progress were revealed at high intensity especially in mild and severe groups. In feces results presented that DAGK, diacylglycerol kinase: MAML2, mastermind like transcriptional coactivator 2: SMC4, structural maintenance of chromosomes 4: TGM2, Transglutaminase 2 were high level in EMN stages both of mild and severe. Interestingly, FOSL1: FOS like 1 can be found only in mild EMN group. Moreover, both of serum and feces samples, the majority of proteins involved in lipid metabolism which plays crucial role for EMN growth, invasive, proliferative and progression. In conclusion, this study demonstrated the occurrence of EMN in Thailand and the specific proteins to diagnostic EMN was discovered

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Attawit Kovitvadhi

Role: Thesis Advisor

Name: Sittiruk Roytrakul

Role: Thesis CO-Advisor

Name: Wanwipa Vongsangnak

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-26

Issued: 2025-07-26

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/parichart-tes-all.pdf

CallNumber: SF951 .P27

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Animal Health and Biomedical Sciences

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	parichart-tes-all.pdf	2.7 MB

ใช้เวลา

0.033874 วินาที