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Kornkanok Sritabtim. In vitro culture of primordial germ cells from Japanese quail (coturnix japonica) embryo. Master's Degree(Animal Health and Biomedical Sciences). Kasetsart University. Office of the University Library. : Kasetsart University, 2023.
In vitro culture of primordial germ cells from Japanese quail (coturnix japonica) embryo
Name: Kornkanok Sritabtim
Classification :.LCCS: QL696.G27
LCSH:
Tissue culture
LCSH:
Germ cells
Abstract:
The Japanese quail (Coturnix japonica) is one of farm animals with high economic values and has been used extensively as avian model for research. Many attempts have been investigated to cryopreserve the quail breeds. Germline chimera technology by using primordial germ cells (PGCs) is possible in quails. Success isolation of PGCs from blood and gonads have been reported, however, its optimal cultivation protocol remained to be elucidated. In this study, we have characterized cultivation of pregonadal PGCs isolated from embryonic parts including, blood (cPGCs), whole embryonic tissues (tPGCs), part of tail-buds (tbPGCs), and mixture of blood and tail-bud tissues (ctbPGCs). The results showed that the cultivation system required a presence of specific embryonic cells as a feeder for quail PGCs, and such system initiated more successfully (as shown in percentage of isolation and cultivation) in tbPGCs (100%, 100%), tPGCs (60%, 55%), and ctbPGCs (60%, 30%), respectively, but not in cPGCs (0%) cultured on mitomycin treated quail feeder cells. Once the co-culture system established, PGCs can be propagated for at least 5 months. These PGCs expressed germ cell specific markers (DAZL and CVH) and have ability to colonize at embryonic gonads. Conclusively, pregonadal PGCs isolated and long term cultivated in vitro requires a unique embryonic feeder and indispensable of proliferating PGCs. Characterization of cell signalings sustained a mutual interaction between PGCs and feeder cells will elucidate an optimal environment of in vitro cultivation in a xenofree condition.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Role:
Thesis Advisor
Role:
Thesis CO-Advisor
Created:
2023
Modified:
2025-07-25
Issued:
2025-07-25
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2566/kornkanok-sri-all.pdf
CallNumber:
QL696.G27 .K67
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Animal Health and Biomedical Sciences
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.030339 วินาที
Kornkanok Sritabtim
| Title | Contributor | Type |
|---|---|---|
| In vitro culture of primordial germ cells from Japanese quail (coturnix japonica) embryo
มหาวิทยาลัยเกษตรศาสตร์ Kornkanok Sritabtim | Kannika Siripattarapravat | วิทยานิพนธ์/Thesis |
Kannika Siripattarapravat
| Title | Creator | Type and Date Create |
|---|---|---|
| Isolation and characterization of canine brain-derived neural stem cells
มหาวิทยาลัยเกษตรศาสตร์ Kannika Siripattarapravat ;Theerapol Sirinarumitr ;Chanin Tirawattanawanich | Napat Nuttonmanit | วิทยานิพนธ์/Thesis |
| Immunohistochemistry for diagnosis and typing of canine intraocular and adnexal lymphoma
มหาวิทยาลัยเกษตรศาสตร์ Aree Thayananuphat;Kannika Siripattarapravat | Natthanet Sritrakoon | วิทยานิพนธ์/Thesis |
| In vitro culture of primordial germ cells from Japanese quail (coturnix japonica) embryo
มหาวิทยาลัยเกษตรศาสตร์ Kannika Siripattarapravat | Kornkanok Sritabtim | วิทยานิพนธ์/Thesis |
| Cryoconservation management through tissue primordial germ cells and a perspective of reprogrammed somatic cells in avian species
มหาวิทยาลัยเกษตรศาสตร์ Kannika Siripattarapravat | Suparat Chaipipat | วิทยานิพนธ์/Thesis |
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