Investigation of light emitting diode effects on bioactive compound accumulation in mulberry

Name: Jiralapat Thamrongwatwongsa

LCSH: Kasetsart University -- Theses. M.S. (Biochemistry) 2021

LCSH: Kasetsart University. -- Department of Biochemistry -- Theses

LCSH: Mulberry

LCSH: Plant bioactive compounds

LCSH: Bioactive compounds

LCSH: Light emitting diodes

Abstract: Mulberry (Morus spp.) is being exploited mainly by sericulture, and its uses also extend to the food, pharmaceutical, and cosmetic industries. Mulberry extracts are rich in many bioactive compounds that exhibit various biological properties. Mulberroside F (Moracin M-6, 3'-di-O-β-D-glucopyranoside), one of the bioactive compounds found in mulberry, has previously been reported as a whitening agent by inhibiting melanin production and showed antioxidant properties. Recently, light-emitting diodes (LEDs) have been widely used as a light source in plant growing systems. Therefore, the objectives of this research were to determine the content of mulberroside F in different parts of the mulberry, investigate the impact of LEDs treatment on mulberroside F accumulation, and study some related biological properties. Firstly, five different types of solvent (ethanol, methanol, ethyl acetate, hexane, and water) were used to extract bioactive compounds, and 60% ethanol was chosen. Different parts of mulberry plants from soil-grown and tissue culture systems were then collected, subsequently extracted, and used for HPLC analysis. The highest content of mulberroside F was found in the root of tissue-cultured plants (207.35 ± 23.42 µg g-1DW), followed by callus (160.97 ± 17.08 µg g-1 DW), root (121.52 ± 6.59 µg g-1 DW), stem bark (37.94 ± 3.90 µg g-1 DW), and leaf (30.85 ± 5.98 µg g-1DW) of soil-grown plants. Next, the plants cultured in vitro were exposed to different LEDs (red, blue, and white) for seven days under controlled conditions (PPFD - 100 µmol m-2 s -1 ; 16/8 h photoperiod; 25 ± 2 °C). The results showed that mulberroside F was significantly increased in the root of plants treated with red and blue LEDs compared to those kept under white LEDs and fluorescent lights (control). Furthermore, the mulberroside F content was positively correlated with the inhibitory effects on tyrosinase activity. Cell viability assay revealed that the root extract does not affect the epidermis carcinoma cell line (A-431). Nevertheless, the human keratinocyte cell line (HaCat) was also unchanged, indicating that the root extracts have less cytotoxicity on cells. Taken together, this study suggested that mulberroside F has potential future utilization in cosmetic industries, and the LEDs system could be used to enhance the production of mulberroside F.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Wannarat Phonphoem

Role: Thesis Advisor

Name: Nattanan T-Thienprasert

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-24

Issued: 2025-07-24

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/jiralapat-tha-all.pdf

CallNumber: QK495.M73

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Kasetsart University

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