Effect of different extracting solvents on antioxidant activity and inhibitory effect on diabetic enzymes of chlorella vulgaris and spirulina platensis

Name: Agustiar, Akhmad Awaludin

LCSH: Kasetsart University -- Theses. M.S. (Fishery Science and Technology) 2022

Classification :.LCCS: QK568.M52

LCSH: Kasetsart University. -- Faculty of Fisheries -- Theses

LCSH: Microalgae

LCSH: Chlorella

LCSH: Spirulina.

LCSH: Antioxidants

LCSH: Diabetes

LCSH: Bioactive compounds

LCSH: Dietary supplements

Abstract: This study aimed to investigate the effect of solvent used in microalgae extraction on bioactive compound composition, antioxidant, and anti-carbohydratehydrolyzing enzyme activities of the crude extracts. Chlorella vulgaris and Spirulina platensis biomass were extracted using different types of solvent, namely n-hexane, ethanol, methanol, and distilled water. The correlation of total phenolic contents (TPC) and total flavonoid contents (TFC) with the antioxidant and αamylase and α-glucosidase inhibitory activities were also evaluated. The results indicated that the methanolic extracts of C. vulgaris and S. platensis had the highest TPC (40.62 ± 4.13 and 64.38 ± 7.39 mg GAE/g, respectively). The methanolic extract of C. vulgaris and S. platensis exhibited the highest antioxidant activity with the IC50 for DPPH of 117.62 ± 4.46 and 97.89 ± 4.64 µg/mL, respectively, and ABTS of 102.52 ± 2.50 and 46.21 ± 0.88 µg/mL, respectively. Furthermore, they strongly inhibited α-amylase activity with the IC50 of 262.97 ± 9.61 and 275.46 ± 12.44 µg/mL, respectively, and α-glucosidase activity with the IC50 of 207.09 ± 6.97 and 169.01 ± 3.16 µg/mL, respectively. TPC was found to have a significant positive correlation with antioxidant activities (DPPH and ABTS) and αglucosidase inhibition activity. Meanwhile, only α-amylase inhibitory activity had a significant positive correlation with TFC. This work indicates that the crude methanolic extract of C. vulgaris and S. platensis are potential sources of natural antioxidants and anti-diabetic food supplements.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Tirawat Rairat

Role: Thesis Advisor

Name: Mingyong Zeng

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-20

Issued: 2025-07-20

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/akhmad-awa-all.pdf

CallNumber: QK568.M52 .A39

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Fishery Science and Technology

Grantor: Kasetsart University

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