แจ้งเอกสารไม่ครบถ้วน, ไม่ตรงกับชื่อเรื่อง หรือมีข้อผิดพลาดเกี่ยวกับเอกสาร ติดต่อที่นี่ ==>
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
Norrachon Sakarat. Quantification of biologically active pyranocoumarins in clausena excavata using quantitative NMR (qNMR). Master's Degree(Chemistry). Kasetsart University. Office of the University Library. : Kasetsart University, 2021.
Quantification of biologically active pyranocoumarins in clausena excavata using quantitative NMR (qNMR)
Name: Norrachon Sakarat
Classification :.LCCS: QK495.R98 .N67
LCSH:
Rutaceae
LCSH:
Pyran
LCSH:
Coumarins
LCSH:
Phytochemicals
LCSH:
Bioactive compounds
Abstract:
A rapid method for the quantification of biologically active pyranocoumarins, clausenidin, dentatin, and nor-dentatin, in Clausena excavata, has been successfully developed. Unlike the traditional HPLC method, the quantitative NMR method (qNMR) employs the quantitative comparison of the peak areas from compounds of interest and an internal standard. The qNMR was performed on plant materials collected from different geographical locations, including Chumphon, Songkhla (Southern region), Kanchanaburi (Western region), Lampang (Northern region), Surin (Northeast region) and Trat (Eastern region). The results indicated that the pyranocoumarins mentioned above are the major components in Clausena excavata. The method exhibits excellent precision, and requires short sample preparation time. The highest level of clausenidin was found in plant materials collected from Songkhla province, while the lowest amount was found in the sample from Lampang province. The amounts of clausenidin from qNMR experiments of samples collected from Songkhla, Kanchanaburi, Chumphon, Trat, Surin, and Lampang are 0.35, 0.32, 0.22, 0.13, 0.10 0.06 percent by weight of the dry plant, respectively. Samples from Surin province contained the highest amount of dentatin and nor-dentatin, and the lowest amount of these two pyranocoumarins was identified from in plant materials collected from Trat province. Besides, the quantities of dentatin and nor-dentatin of Surin, Lampang, Songkhla, Kanchanaburi, Chumphon, and Trat are 3.02, 1.70, 1.14, 0.48, 0.40, 0.17, and 3.41, 1.96, 1.57, 0.57, 0.34, 0.14 percent by weight of the dry plant, respectively. The qNMR method developed herein was verified by the traditional HPLC method. The results indicated good agreement between the two methods. Therefore, the qNMR technique could be further developed to quantify various biologically active compounds of natural sources.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Pitak Chuawong
Role:
Thesis Advisor
Name: Pimpa Hormnirun
Role:
Thesis CO-Advisor
Name: Tharinee Saleepochn
Role:
Thesis CO-Advisor
Created:
2021
Modified:
2025-07-20
Issued:
2025-07-20
วิทยานิพนธ์/Thesis
application/pdf
URL: http://www.lib.ku.ac.th/KUthesis/2564/norrachon-sak-all.pdf
CallNumber:
QK495.R98 .N67
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Chemistry
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.0432 วินาที
Norrachon Sakarat
| Title | Contributor | Type |
|---|---|---|
| Quantification of biologically active pyranocoumarins in clausena excavata using quantitative NMR (qNMR)
มหาวิทยาลัยเกษตรศาสตร์ Norrachon Sakarat | Pitak Chuawong | วิทยานิพนธ์/Thesis |
Pitak Chuawong
Pimpa Hormnirun
Tharinee Saleepochn
| Title | Creator | Type and Date Create |
|---|