The selection of genetic stable lines from somaclonal variants chrysanthemum and the incidence of DNA methylation

Name: Adisak Kaewkam

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Biotechnology) 2021

Classification :.LCCS: QK495.C74

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Chrysanthemums -- Genetics

LCSH: Chrysanthemums -- Mutation breeding

LCSH: Chrysanthemums

LCSH: Methylation

LCSH: Tissue culture

Abstract: In previous research, the chrysanthemum lines derived from a somaclonal variation of two commercial cultivars, ‘White Button’ (button type flower with only white ray florets), and ‘Polaris Pink’ (decorative type flower with pink ray florets) were obtained. The variant lines of ‘White Button’ cultivars demonstrated the variation in flower form that altered from button type to daisy type flower with 3 sub-types of variation in the positions and number of ray and disc florets. In ‘Polaris pink’, the change in color of ray florets from pink to light purple was observed. However, all of these variants were chimera. Thus, in order to extract the solid variant lines, several generations of single-node cuttings proof to be effective. For this procedure, tissue culture could aid to shorten the time. In this experiment, the selected chimeric plants were surface sterilized and cultured on solid MS medium and single node tissue were transferred onto a new medium every month for eight generations. Plantlets were transplanted in the experimental field and morphological characterization was performed at the full bloom stage. The result exhibited that, in the ‘White Button’ cultivar, in the first field grown of V8 generation, there were only four solid variant lines observed from 75 plants evaluated. However, the study in the second field grown of V16 generation revealed higher percentage of solid variant line of 24 out of 135 plants observed. However, segregation still be observed in the progenies indicated that the solid variants could not be extracted even sixteen generations of single node cuttings applied. In contrast, in ‘Polaris Pink’ cultivar, the solid mutant lines could be extracted since V8 generation as all plantlets tested in this generation were uniformed. The determination of DNA methylation involved in somaclonal variation in the first field grown plants using MSAP technique revealed the incidence of DNA methylation involved with somaclonal variation occurrence. The somaclonal variant lines in ‘White Button’ tended to have demethylation events. On the other hand, in ‘Polaris Pink’ cultivar, the more methylation events observed in the variant line than that of the control line.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Sermsiri Chanprame

Role: Thesis Advisor

Created: 2021

Modified: 2025-07-20

Issued: 2025-07-20

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/adisak-kae-all.pdf

CallNumber: QK495.C74 .A35

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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