Molecular screening for Amylose content, gel consistency, and gelatinization temperature in landrace Rice varieties

Name: Um, Dalin

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Resources) 2019

Classification :.LCCS: SB191.R5

LCSH: Kasetsart University. Department of Agricultural Resources -- Theses

LCSH: Rice -- Breeding

LCSH: Genetic markers

Abstract: Amylose Content (AC), gel Consistency (GC), and gelatinization temperature (GT) are the most important physiochemical charateristics of starch which influence on eating and cooking quality of rice. Landrace rice the traditional varieties of rice, grown by farmers and passed down from generation to generation. It represents a unique and critical source of genetically variable traits that can serve as a resource of future rice improvement. As variation of genetic in landrace rice is beneficially to reveal so microsatellite markers, single nucleotide polymorphism (SNP), and a cleaved amplified polymorphic sequence (CAPS) marker (RM190F/GBSSW2R/AccI) were used to identified the allelic variance of waxy gene as well as soluble starch synthase (SS) locus in sixty (60) landrace rice varieties. The AC of landrace rice ranged from 3.29-23.72%. Most of rice accessions were very low and low amylose content and only 8 accessions were intermidiate amylose content. GC was found in ranged of 20-150 mm. Medium hard gel was found only in 1 accession, while more than half of accessions were hard gel. Over half of accessions were found in high GT and only 7 accessions were low GT. Marker RM190 produced 5 alleles with 8 homo and heterozygous genetypes. While RM314 produced 4 alleles with 5 homo and heterozygous genotypes. CAPS marker produced 3 genotype groups including GG, TT, and GT. High resultion melting (HRM) analysis of SSIIa gene generated by real-time PCR produced 2 different groups of rice accession with the melting temperature of 84.3- 84.7oC and 85.2-85.35oC. Three alleles of SSI gene were detected in which almost accessions were allele 1 and each allele 2 and allele 3 had 1 accession. The unweight-pair group method with artihmetic mean (UPGMA) cluster analysis was performed for construting genetic relationship among rice samples. Four genetic clusters based on RM190 seperated the accessions linked to allele 2 and genotype 2/2 associated to low amylose content. Three cluster groups were also found for RM314. Cluster I possessed in almost rice accessions. Only 3 and 5 accessions were found in cluster II and cluster III, respectively. CAPS marker illustrated clearly cleavaged pattern in the studied accessions. However,the information of moleclular marker linked to rice cooking and eating quality traits and information regarding to genetic relationship would be useful for rice improvement of eating and cooking quality in the future rice breeding program.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Thanwanit Thanyasiriwat

Role: Thesis Advisor

Name: Phinyarat Kongprakhon

Role: Thesis CO-Advisor

Created: 2019

Modified: 2025-07-17

Issued: 2025-07-17

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/dalin-um-all.pdf

CallNumber: SB191.R5 .U43

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Resources

Grantor: Kasetsart University

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