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Waraporn Bunnak. Biosynthetic studies of menisporopsin a and heterologous expression of unknown polyketide synthase genes from menisporopsis theobromae BCC 4162. Doctoral Degraee(Chemistry). Kasetsart University. Office of the University Library. : Kasetsart University, 2020.
Biosynthetic studies of menisporopsin a and heterologous expression of unknown polyketide synthase genes from menisporopsis theobromae BCC 4162
Name: Waraporn Bunnak
Classification :.LCCS: QP752.P65
LCSH:
Filamentous fungi
LCSH:
Polyketides
LCSH:
Natural products
Abstract:
Transcriptomic results of Menisporopsis theobromae BCC 4162 during the menisporopsin A production phase revealed that four PKS genes from clusters 51, 55, 89 and 93 are possibly involved in the biosynthesis of menisporopsin A. In this work, each PKS gene candidate and a type III PKS gene from cluster 75 were constructed into an expression vector and introduced into the heterologous host, Aspergillus oryzae NSAR1, for metabolite production. Our results showed that no secondary metabolites could be identified from clusters 55, 75 and 93. Only coexpression of NR-PKS gene (men1) from cluster 51 and R-PKS gene (men2) from cluster 89 resulted in the production of (-)-orthosporin, (-)-6-hydroxymellein and ascotrichalactone A. The latter differs from menisporopsin A only in the presence of a keto group in its structure. This indicates that only Men1 and Men2 are sufficient for the multiple esterification and cyclolactonization of menisporopsin A. These reactions could be catalyzed by the thioesterase (TE) domain of Men2 (Men2 TE). In order to study the Men2 TE function, recombinant protein expression in E. coli was performed. The solubility of Men2 TE was improved by joining with either mono or didomain ACP as Men2 ACP2-TE and ACP1-ACP2-TE. The Men2 ACP2-TE was successfully converted to the holo form catalyzed by the phosphopantetheinyl transferase, Sfp. However, its hydrolytic activity is still unclear and need further investigation. In addition, the solution biophysical characterization was also performed on both Men2 ACP2-TE and ACP1-ACP2-TE using CD and SAXS techniques. The latter revealed beads-on-a-string configuration of both proteins providing the first insight into the ACP-TE domain organization in the NR-PKS.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Pakorn Wattana-Amorn
Role:
Thesis Advisor
Name: Pitak Chuawong
Role:
Thesis CO-Advisor
Role:
Thesis CO-Advisor
Created:
2020
Modified:
2025-07-11
Issued:
2025-07-11
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2563/waraporn-bun-all.pdf
CallNumber:
QP752.P65 .W37
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degraee
Descipline: Chemistry
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.02265 วินาที
Waraporn Bunnak
| Title | Contributor | Type |
|---|---|---|
| Biosynthetic studies of menisporopsin a and heterologous expression of unknown polyketide synthase genes from menisporopsis theobromae BCC 4162
มหาวิทยาลัยเกษตรศาสตร์ Waraporn Bunnak | Pakorn Wattana-Amorn | วิทยานิพนธ์/Thesis |
Pakorn Wattana-Amorn
Pitak Chuawong
Supachai Vuttipongchaikij