Biopolymer producing bacteria from soil contaminated battery heavy metal and their immobilization for bioremediation

Name: Puttinai Wechkama

LCSH: Kasetsart University -- Theses. M.S. (Environmental Science and Technology) 2021

Classification :.LCCS: TD192.5

LCSH: Kasetsart University. -- Department of Science -- Theses

LCSH: Bioremediation

LCSH: Biopolymers

LCSH: Soil pollution

LCSH: Electronic waste -- Environmental aspects

LCSH: Soils -- Heavy metal content

LCSH: Heavy metals -- Environmental aspects

LCSH: Electric batteries -- Environmental aspects

Abstract: Heavy metal contamination is an environmental problem affecting public health. To survive, microorganisms have developed mechanisms to protect themselves from heavy metal poisoning. One of the important mechanisms is the formation of biopolymers by secreting an extracellular polymeric substance (EPS). EPS plays a role in adsorption of heavy metals by ions exchange. The aims of research are to isolate and evaluate the heavy metal resistance of biopolymer producing bacteria and to optimize immobilization conditions of biofilm on agricultural waste. Biopolymer producing bacteria was isolated from soil at the electronic waste warehouse and selected the viscous colonies on selective media (MWA). The heavy metal resistance bacteria were evaluated on TSA containing 100 mg/L of lead (Pb) or cadmium (Cd). Eight isolates grew on TSA contaning with Pb while 6 isolates grew on TSA containing with Cd. Most isolates were identified sequencing as genus Bacillus and Pseudomonas base on 16S rDNA. Minimal Inhibitory Concentration (MIC) of Pb and Cd were at 1400 mg/L and 400 mg/L, respectively. Minimum Bactericidal Concentration (MBC) of Pb and Cd were at 1600 mg/L and 400 mg/L, respectively. The optimum conditions for biofilm immobilization were showed that the immobilization of B. cereus DTM 1-1 and B. cereus DTM 1-5 on coconut husk in MWB at pH 6.0 and 35 °C showed the highest of immobilized biomass as a dry weight at 345 ± 4.46 mg/g and 369 ± 6.99 mg/g, respectively. The results of structural analysis of bacterial immobilized on coconut husks by SEM showed that biopolymer producing bacteria had the potential to be immobilized on agricultural waste. Furthermore, biopolymer producing bacteria immobilized on agricultural waste can be used in bioremediation to remediate the heavy metal toxic.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Jintanart Wongchawalit

Role: Thesis Advisor

Name: Thanawan Panich-pat

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-10

Issued: 2025-07-10

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/puttinai-wec-all.pdf

CallNumber: TD192.5 .P88

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Environmental Science and Technology

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	puttinai-wec-all.pdf	1.65 MB

ใช้เวลา

0.037597 วินาที