Suchawadee Loyawattananan. Characterization and application of alternative antimicrobial surfactants on food contact surface. Master's Degree(Food Science). Kasetsart University. Office of the University Library. : Kasetsart University, 2020.
Characterization and application of alternative antimicrobial surfactants on food contact surface
Abstract:
The objective of this research was to investigate the potential of selected surfactants for their physical properties and antibacterial activity in order to find a natural/alternative antibacterial surfactant. Nine commercial surfactants including cocamidopropyl betaine, coco glucoside, disodium laureth sulfosuccinate, lauryl glucoside, sodium cocoyl isethionate, sodium lauroamphoacetate, sodium lauroyl glutamate, Sapindus emarginatus extract, and saponin powder were assessed. Their properties were compared to Spectrum®, a commercial-grade synthetic cleaning and sanitizing agent in a group of quaternary ammonium compounds (Quats) that is widely used by food manufacturers. The pH value, foaming ability, contact angle and surface tension were measured. The selected surfactants were categorized by their pH values into two groups, including acid surfactant and alkaline surfactant. All selected surfactants had foaming ability. Their surface tension values varied according to the type of surfactant. For antimicrobial activity, agar well diffusion was used for screening against 12 strains of foodborne microorganisms including food spoilage and foodborne pathogens. Sodium lauroamphoacetae had the broadest spectrum against tested microorganisms, followed by cocamidopropyl betaine and coco glucoside, respectively. Moreover, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the selected surfactants and timekill profile of coco glucoside and sodium lauroamphoacetae against Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus were investigated. Coco glucoside showed the strongest antibacterial activity, particularly against L. monocytogenes. Besides, coco glucoside showed the ability to reduce L. monocytogenes on stainless steel coupon no. 304 on both clean and unclean surfaces under static washing condition (soaking) and slightly mechanical force washing condition (shaking). 1% coco glucoside inactivated L. monocytogenes (3.92 Log CFU/cm2 ) inoculated on stainless steel coupon within 10 min. Under this condition, scanning electron micrography revealed the lower density of L. monocytogenes on the stainless steel coupon compared to control, while 1% of coco glucoside caused damage leading to cell death. Therefore, coco glucoside has the potential to be an alternative antimicrobial surfactant for application in the food industry and household use.
Kasetsart University. Office of the University Library