Self-assembly of DNA/chitosan supramolecular biomaterial

Name: Pitchaya Pakornpadungsit

LCSH: Kasetsart University -- Theses. M.S. (Nanomaterials Science) 2018

Classification :.LCCS: R857.M3

LCSH: Kasetsart University. -- Department of Materials Science -- Theses

LCSH: Biomedical materials

LCSH: Supramolecular chemistry

LCSH: Chitosan

Abstract: Normally, supramolecular chemistry’s primary part consists of self-assembly, molecular recognition and noncovalent interaction. Self-assembly process can give many types of supramolecular structure using noncovalent interactions. These interactions are important to the fields of materials that can imply molecular recognition of the supramolecular character. This means that we can apply the noncovalent interactions in the design of new materials. DNA is a good example for supramolecular materials because it is able to form supramolecular structure by forming specific hydrogen bonds between its base pairs. In this work, we focused on the preparation and characterization of dried complexes obtained from self-assembly process between anionic DNA and cationic chitosan based on their electrostatic interaction. We varied 5 ratios of the dried complexes, DNA:chitosan ratios were 0.25:1, 0.5:1, 1:1, 1:0.5 and 1:0.25, to better understand the effect of DNA and chitosan content to the properties of the dried complexes. Based on the elemental analysis, DNA/chitosan dried complexes were found to form in a 0.5:1, 1:1 and 1:0.5 stoichiometry. The structure of the dried complexes had large scale (>100nm) structural order. Based-on FTIR analysis, the functional groups of the dried complexes in 5 ratios were not significantly different. Melting temperature of the dried complexes was found at the first DSC scan due to the evaporation of water. This type of temperature trends to increase with the DNA content. Morphology properties show the porous structure in complexes. All the dried complexes in 5 ratios do not generate the toxicity to the Valve endothelial cells. For future work, these types of material could be useful for developing functional material such as a new type of drug delivery material, etc.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Wirasak Smitthipong

Role: Thesis Advisor

Created: 2018

Modified: 2025-07-09

Issued: 2025-07-09

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/pitchaya-pak-all.pdf

CallNumber: R857.M3 .P58

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Nanomaterials Science

Grantor: Kasetsart University

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