Investigation of modified-hydroxyapatite incorporating poly (methyl methacrylate)-grafted-chitosan biocomposite scaffolds for bone tissue engineering

Name: Tanatsaparn Tithito

LCSH: Kasetsart University -- Theses. M.S. (Physics) 2018

Classification :.LCCS: QC173.4.C63

LCSH: Kasetsart University. -- Department of Physics -- Theses

LCSH: Composite materials

LCSH: Tissues -- Mechanical properties

LCSH: Tissue engineering

LCSH: Tissue scaffolds

LCSH: Biomedical materials

LCSH: Calcium phosphates

LCSH: Bone regeneration

LCSH: Hydroxyapatite

Abstract: Bionanocomposites scaffolds having the proper mechanical properties and improved biological performances would be very beneficial for bone repairing applications. In the present study, composite scaffolds of chitosan-graft-poly (methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded calcium phosphate of hydroxyapatite (mHA) (obtained by the hydrothermal treatment of HA) (mHA@Chi-g-PMMA) were prepared by the blending method. Their capabilities to sustained drug and protein release were also studied. Physicochemical analysis showed a successful incorporation of mineral ions in HA particle and a better distribution of the mHA in the Chi-g-PMMA polymer matrix during the in situ processing. Physical properties such as average porous was 65.74%, the compressive strength and the young modulus were 14.01 and 585.67 MPa, respectively. In vitro experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold were done. They revealed that the synthesized scaffold had been improved in their cell attachment, viability and proliferation after 7 days of culturing. As a delivery vehicle, the in vitro releases of the anti-cancer drug (doxorubicin (DOX)) and of the protein (bovine serum albumin (BSA)) were studies. Both biomolecules were mix and it was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation. These experimental results demonstrated that the bionanocomposite of mHA@Chi-g-PMMA scaffold can be utilized as a temporal scaffold for bone cells ingrowth and also be used for drug delivery during bone repairing.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Weeraphat Pon-On

Role: Thesis Advisor

Created: 2018

Modified: 2025-07-08

Issued: 2025-07-08

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/tanatsaparn-tit-all.pdf

CallNumber: QC173.4.C63 .T36

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Physics

Grantor: Kasetsart University

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