Watcharin Phoomkhong. Discriminating lethal conceptrations for pyrethroid susceptibility monitoring and behavioral responses to synthetic repellents against anopheles epiroticus, a Malaria Vector in Thailand. Master's Degree(Entomology). Kasetsart University. Office of the University Library. : Kasetsart University, 2018.
Discriminating lethal conceptrations for pyrethroid susceptibility monitoring and behavioral responses to synthetic repellents against anopheles epiroticus, a Malaria Vector in Thailand
Abstract:
Anopheles epiroticus is a malaria vector in Thailand found primarily along coastal areas with brackish water habitats. Insecticides, particularly pyrethroid class compounds, are commonly used to control malaria vectors in Thailand. The lack of specific discriminating lethal concentrations for An. epiroticus has possibly compromised a more accurate assessment of physiological susceptibility to various chemicals. The routine assessment of vector response to insecticides is a key program management component to prevent or mitigate the development of resistance. The purpose of this study was to determine the discriminating (diagnostic) lethal concentrations of five common synthetic pyrethroids (deltamethrin, permethrin, bifenthrin, lambda-cyhalothrin, and alpha-cypermethrin) used in Thailand for malaria control, against a susceptible colonized population of An. epiroticus and observations on the responses of An. epiroticus to synthetic mosquito repellents (DEET, IR3535) and three synthetic pyrethroids (deltamethrin, permethrin, alpha-cypermethrin) using an excito-repellency test system. Final discriminating concentrations were 0.006% deltamethrin, 0.349% permethrin, 0.033% bifenthrin, 0.012% lambda (0)-cyhalothrin, and 0.0009% alpha-cypermethrin. Using concentrations established for each chemical, a field population of An. epiroticus from southern Thailand was found completely susceptible to each concentration. The wild-caught populations of An. epiroticusfrom Songkhla population exhibited the strong excitatory effect in both contact irritancy and non-contact repellency to DEET and IR3535. These findings indicate that contact irritancy is the primary behavioral response of deltamethrin, -cypermethrin and permethrin. Periodic monitoring of insecticide susceptibility of An. epiroticus and other malaria vector species is needed to assess the efficacy of chemicals and guide insecticide policy and control programs.
Kasetsart University. Office of the University Library