Cytotoxicity effects of cratoxylum formosum against hepatitis B virus in cancer cells and utilisation of Cratoxylum formosum in green synthesised zinc oxide nanoparticles

Name: Damita Jevapatarakul

LCSH: Kasetsart University -- Theses. M.S. (Biochemistry) 2020

Classification :.LCCS: QR201.H46

LCSH: Kasetsart University. -- Department of Biochemistry -- Theses

LCSH: Hepatitis B virus

LCSH: Hepatitis viruses

LCSH: Liver cancer

LCSH: Liver

Abstract: Chronic hepatitis B virus (HBV) infection is the main cause of Hepatocellular carcinoma (HCC). Edible plants such as Curcumin longa Linn and Cratoxylum formosum have been consumed as a traditional medicine for curing liver diseases and cancers. However, combination effects of C. longa and C. formosum have not been reported. Nevertheless, the utilization of C. formosum leaves extract to synthesise zinc oxide nanoparticles (ZnO NPs) has also never been reported. This study therefore focused to investigate effects of combined extracts between C. longa and C. formosum on the anti-liver and anti-HBV activities, including alteration of protein and miRNA expressions in liver cancer cells. The physical and biological properties of ZnO NPs from C. formosum green synthesis were also examined. Cytotoxicity effects of crude extracts on liver cancer cells were examined by MTT assay and showed that a single C. formosum, and combined extracts could significantly inhibit HepG2.2.15 (liver cancer cells with stabilising HBV replication). Moreover, results from quantitative real-time PCR indicated that at 150 µg/mL of the combined extracts specifically suppressed HBx gene expression. Furthermore, Western blot analysis demonstrated that crude extracts could up-regulate p53 protein, while reducing NF-κB level. The expressions of miR-34a-3p and miR-199a/b-3p were significantly up-regulated by C. longa extract, while combined extracts only increased expression of miR-34a-3p. Lastly, the green-synthesised ZnO NPs showed anti-bacterial and anti-non-melanoma skin cancer cells (A431) activities. This study firstly reported that combined C. longa and C. formosum extracts possessed anti-liver activity and anti-HBx gene expression including alteration of miRNAs expression. Additionally, ZnO NPs could be synthesised by using C. formosum as utiliser with the potential activities for therapeutic applications

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Nattanan T-Thienprasert

Role: Thesis Advisor

Name: Chotika Yokthongwattana

Role: Thesis CO-Advisor

Created: 2020

Modified: 2025-07-07

Issued: 2025-07-07

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2563/damita-jev-all.pdf

CallNumber: QR201.H46 .D35

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Kasetsart University

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