Aerobic process for removal of organic pollutants from contaminated harbor sediment

Name: Sudarat Jaisa-Ard

LCSH: Kasetsart University -- Theses. M.Eng. (Environmental Engineering) 2018

Classification :.LCCS: TD756.45

LCSH: Kasetsart University. -- Department of Environmental Engineering -- Theses

LCSH: Sewage -- Purification -- Anaerobic treatment

LCSH: Sewage -- Purification -- Biological treatment

LCSH: Polycyclic aromatic hydrocarbons

Abstract: The aim of this study was to investigate the possibility of applying mixed culture bacteria prepared from contaminated soil to remove COD and PAHs in the sediment. The bacteria mixture consists of three specific diesel-degradable bacteria including Achromobacter insolitus, Candida spp. and Xanthobactor polyaromatici yorans. The experiments were carried out with 3 conditions, i.e. harbor sediment only, harbor sediment with mixed culture bacteria and harbor sediment with mixed culture bacteria and synthetic nutrient solution. After 7 days, treated solution was withdrawn from the reactor through MF hollow fiber membrane while the microorganisms were retained. The next experiment was then proceeded by adding new harbor sediment and/or nutrient solution depending on the experimental condition. The indigenous microorganisms in harbor sediment could remove soluble fraction of COD by 14.24% and almost all studied PAHs. Highest removal was observed for anthracene (An) at 92.05%. The results also reveal that the presence of mixed culture bacteria could slightly improve the removal of SCOD to 20.15% and benzo[b]fluoranthene (BbF) was highest removed at 86.19%. The addition of nutrient solution helped removing high molecular PAHs, i.e. chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF) at 21.29, 42.21, and 61.38% respectively. The cytotoxicity assay of treated sediment on cell viability of normal cells (Clone 9) yielded highest normal cells viability (71.73%) under the presence of harbor sediment only whereas 93.16% cancer cells viability was highest achieved under the presence of harbor sediment and with mixed culture bacteria and nutrient solution.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chart Chiemchaisri

Role: Thesis Advisor

Name: Wilai Chiemchaisri

Role: Thesis CO-Advisor

Created: 2018

Modified: 2025-07-05

Issued: 2025-07-05

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/sudarat-jai-all.pdf

CallNumber: TD756.45 .S83

eng

DegreeName: Master of Engineering

Level: Master's Degree

Descipline: Environmental Engineering

Grantor: Kasetsart University

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