Phitchaya Jaemwimol. Inactivation of tilapia lake virus by common aquaculture disinfectants. Master's Degree(Veterinary Microbiology). Kasetsart University. Office of the University Library. : Kasetsart University, 2019.
Inactivation of tilapia lake virus by common aquaculture disinfectants
Abstract:
Tilapia lake virus (TiLV) is a cause of critical fish viral disease with high mortality and economic losses. Virucidal effects on TiLV of the following five disinfectants (active ingredients : 2.5 ppm iodine, 10 ppm sodium hypochlorite (NaOCl), 300 ppm hydrogen peroxide (H2O2), 80 ppm formalin and 5,000 ppm (0.5%) Virkon®) were investigated. All affecting factors on the disinfectants efficacy, including temperature, contact time and soiling (organic matter) interference, were also examined under natural aquaculture mimicking conditions. The results showed that TiLV was successfully inactivated with more than 5 log10 TCID50 ml−1 by all disinfectants except formalin when incubated for 10 min at 28°C. Similar inactivation levels on TiLV when incubated with NaOCl and Virkon® for 10 min at 4°C were observed. After extended exposure of TiLV with formalin from 10 to 60 min at 28°C, the results showed improving on TiLV inactivation with more than 5 log10 TCID50 ml−1. The mimicking soiling interference by increasing synthetic organic matter in the water has shown to reduce the efficacy of NaOCl, iodine and H2O2 under 28°C; 10 min. However, Virkon® still effective with more than 5 log10 TCID50 ml−1 inactivation. This study suggested that most common disinfectants could effectively reduce viral loads to minimum levels. To control the spread of TiLV in aquaculture farms and related facilities, the appropriate use of such disinfectants should be concerned.
Kasetsart University. Office of the University Library