Colistin resistant escherichia coli from swine farms and retail pork

Name: Pramualchai Ketkhao

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Biotechnology) 2019

Classification :.LCCS: SF977.E83

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Escherichia coli infections in swine

LCSH: Escherichia coli

LCSH: Swine -- Infections

Abstract: The 206 E. coli from four swine farms that had different farm management and colistin usages were studied for colistin minimum inhibitory concentration and mcr-1 and mcr-2 plasmid mediated colistin resistant genes. The isolates were cultured by longitudinally sampling at three stages of swine production cycle comprising piglet, fattening and before slaughtering. Two intensive farms that used colistin as feed medicated showed the high colistin resistance rates at 84.6% and 58.1%. While one intensive farm that did not used colistin showed the low colistin resistance rate at 14.3%. The other farm is a small natural swine farm without antimicrobial usage showed the colistin resistance rate at 41.8%. Most of colistin resistant isolates had MIC at 8 µg/ml (49%), followed by ≥16 µg/ml (48%) and 4 µg/ml (3%). Among 100 colistin resistant E. coli, mcr-1 and mcr-2 were detected at 64% and 38%, respectively. The detection rates of mcr-1 and mcr-2 were not significantly different among the farms (P >0.05). Though there were so many factors involving with antimicrobial resistances, our result confirmed that colistin use with feed medicated in intensive farms could significantly impact to the higher colistin resistance rates (P <0.05). However, the factors of colistin resistance in the natural swine farming should be investigated more. In addition, the same study was also performed in 52 E. coli isolated from pork (n=32) and environment (n=28) at six retail butcher shops in Bangkok. Fortunately, the only one isolate from pork was resistant to colistin and it did not carry either mcr-1 or mcr-2. Furthermore, the study of plasmid transferability found that mcr-1 and mcr-2 harbored plasmids were successfully transferred into recipient E. coli by both conjugation and transformation. The differences of colistin resistance profiles and their resistant genes in swine farms and retail markets might be helpful information for AMR surveillance program along pork production processes.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Patamaporn Amavisit

Role: Thesis Advisor

Name: Sukanya Thongratsakul

Role: Thesis CO-Advisor

Created: 2019

Modified: 2025-07-03

Issued: 2025-07-03

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/pramualchai-ket-all.pdf

CallNumber: SF977.E83 .P75

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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