Genetic diversity of ty1-copia retrotransposons and applicability of inter-primer binding site amplification in Nymphaea species

Name: Runch Tuntipaiboontana

LCSH: Kasetsart University -- Theses. M.S. (Genetic Engineering) 2018

Classification :.LCCS: QK495.N97

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Water lilies -- Genetic engineering

LCSH: Nymphaea

LCSH: Nymphaeaceae

LCSH: Genetic markers

LCSH: DNA

Abstract: For their exceptional beauty and great variety, waterlilies of the genus Nymphaea are popular ornamental plants worldwide. Molecular markers have become standard practices in the waterlily breeding for accurate variety identification. With knowledge about transposable elements, retrotransposon-based markers have been developed for various plant species. Due to the lack of information about retrotransposons in waterlilies, two experiments were conducted in this study. The first experiment was performed to confirm the presence and analyze the diversity of the Ty1-copia retrotransposons in waterlilies. 133 sequences of a conserved domain in the reverse transcriptase gene of Ty1-copia elements were isolated from 14 waterlily varieties and found to be homologous to either Ale, Angela, or TAR lineages. Sequences homologous to the Ale lineage were found to be abundant and diverse. Those homologous to the Angela lineage were found to be very conserved, but scarce. Those homologous to the TAR lineage were relatively conserved and were found in ample amounts. Furthermore, the results indicate that those homologous to the Angela or TAR lineages are more conserved to their counterpart in other plants than those homologous to the Ale lineage. The second experiment was performed to determine the applicability of iPBS amplification as a method for producing DNA fingerprints in waterlilies. The amplification using each iPBS primers produced multiple DNA fragments as expected. 33 DNA fragments produced were sequenced and analyzed. It was unable to determine if the fragments contain the expected conserved regions. Some fragments also contain conserved domains that contradicts with the expectation. Thus, it is unable to confirm if the method is applicable in Nymphaea species.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Vipa Hongtrakul

Role: Thesis Advisor

Name: Sompid Samipak

Role: Thesis CO-Advisor

Created: 2018

Modified: 2025-07-02

Issued: 2025-07-02

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/runch-tun-all.pdf

CallNumber: QK495.N97 .R86

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Genetic Engineering

Grantor: Kasetsart University

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