Production of bioethanol, fuAfrasiab Khanntioxidant compounds from oil palm trunk in biorefinery process

Name: Tareen, Afrasiab Khan

LCSH: Kasetsart University -- Dissertations. Ph.D. (Biotechnology) 2020

Classification :.LCCS: TP248.65.L54

LCSH: Kasetsart University. -- Department of Biotechnology -- Dissertations

LCSH: Lignocellulosic

LCSH: Cellulose

LCSH: Lignin

LCSH: Ethanol -- Production

LCSH: Antioxidants

LCSH: Oil palm -- Utilization

Abstract: A biorefinary concept of cellulose ethanol by a simultaneous saccrification and fermentation process was investigated in this research. Initially, hemicellulose removal of oil palm trunk (OPT) by steam explosion pretreatment was operated at 210°C for 4 minutes. For the catalytic conversion of steam exploded hydrolysate to produce furfural by using sulfuric acid, amberlyst-15 and a combination of hydrotalacite and amberlyst-15. The maximum xylose conversion: 100% and furfural yield: 66.43% were achieved at 170°C for 2 hrs of combinatorial effect of sulfuric acid and dimethylfuramide. Whereas, coupled effect of hydrotalacite and amberlyst-15 exhibited highest xylose conversion: 88.12%; furfural yield: 40.88% and selectivity: 46.39% at 110°C for 3h. Furfural yield and selectivity were witnessed to be declined with intensification in temperature in the presence of both acid and base catalysts. The alkaline hydrogen peroxide (AHP) as a pretreatment was studied to remove lignin and increase enzymatic digestibility of OPT for production of biofuels and phenolic compounds in biorefinery processes. The effect of H2O2 concentration (1%-5%) temperature (50-90 °C) and time (30-90 min) were studied to find out the optimum condition for lignin removal. The optimum condition attained at 70 °C, 30 min, and 3% H2O2 g /g of biomass, not only increased the cellulose content from 38.67% in raw material to 73.96%, but also removed lignin and hemicellulose up to 50% and 57.12%, respectively. The results of both SEM and FT-IR showed disruption and changes in OPT structure and transformation/dissolution of lignin and hemicellulose molecules. The black liquor obtained from AHP treatment, contained about 5.13 mg gallic acid equivalent (GAE)/g dry sample of total phenolic content (TPC) and an antioxidant activity of 59.80% and 65.51% inhibition by DPPH and ABTS assays, respectively. A simultaneous saccharification and fermentation (SSF) was used to produce ethanol by using AHP pretreated fibers as substrate. Initially, SSF was carried out with both batch and fed batch mode in a total volume of 300 mL. In Batch SSF the highest ethanol concentration (CP) was 33.11 g/L, ethanol productivity (QP) 0.5501 g/L/h, ethanol yield (YP/S) 0.4043 g/g and theoretical ethanol yield 82.09%, similar results were observed when ethanol production was studied with feeding strategy in bioreactor with working volume of 1 liter. In addition, the pre-hydrolysis and simultaneous saccharification and fermentation (PSSF) gave the ethanol concentration (CP) 33.28 g/L and theoretical ethanol yield 77.81%. It was revealed that PSSF did not enhance the efficiency of bioethanol production but reduce the overall fermentation time.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Pramuk Parakulsuksatid

Role: Thesis Advisor

Name: Vittaya Punsuvon

Role: Thesis CO-Advisor

Created: 2020

Modified: 2025-06-30

Issued: 2025-06-30

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2563/afrasiab-kha-all.pdf

CallNumber: TP248.65.L54 .T37

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Biotechnology

Grantor: Kasetsart University

©copyrights Kasetsart University

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