Response and acclimation to drought and heat stress on dendrobium sonia Earsakul and protoplast transient expression system for genome editing studies in dendrobium

Name: Thanachok Taticharoen

LCSH: Kasetsart University -- Dissertations. Ph.D. (Agricultural Sciences) 2022

Classification :.LCCS: QK495.O64

LCSH: Kasetsart University. Faculty of Agriculture at Kamphaeng Saen -- Dissertations

LCSH: Dendrobium -- Genetic

LCSH: Dendrobium -- Drought tolerance

LCSH: Dendrobium -- Effect of stress on

LCSH: Orchids -- Genetics

LCSH: Orchids -- Drought tolerance

LCSH: Orchids -- Effect of stress on

LCSH: CRISPR (Genetics)

Abstract: The study of CAM response to the drought is well studied, however, response to high temperature and both stresses combination are still limited. We found that dendrobium Sonia ‘Earsakuĺshowed a very high tolerance to drought stress by having pseudobulb organ acting as a reservoir to overcome long-term drought period. The acclimation mechanism was minimizing the stomata opening time only at optimum duration (phase II; 04.00-06.00) to reduce the water loss by transpiration, furthermore, PEPC activity increased when facing drought to capture internal CO2 during stomata closed. While hightemperature stress, in the long term, plants can recover and acclimate to high-temperature stress by opening stomata wider and longer to minimize the effect of heat stress. Aerenchyma structure was found in pseudobulb tissue which believed it helps alleviate high-temperature stress. The stress combination showed the severest symptom both physical and CER since 7 DAT. The acclimation mechanism showed the same pattern as drought stress but was unable to soothe the heat stress due to the limitation of water. From the response to the stresses, minimizing the irrigation water for Dendrobium production is required. The reduction of irrigation was done by either time reduction or changing the irrigation head to a mini springer head and applying irrigation at a different time of the day. We found that the adjusted irrigation method can reduce water consumption up to 71 % compared with regular irrigation. The reduction of water during the dry season did not affect both growth and yield in long-term irrigation applications. Irrigation during dawn and evening time when CAM plants open stomata showed higher total leaf area, leaf fresh and dry weight compared with common irrigation time of farmer which is during the morning till noon when CAM plant stomata closed. For further perspective in terms of breeding technology, CRISPR/Cas9 genome editing is a powerful tool that can achieve the goal by consuming a shorter time. Protoplast transformation by PEG-4000 can shorten the time of testing the selected vector. Our selected pRGEB32 showed high efficiency and found genome editing in the protoplast of Dendrobium Formidible which is able to test in PLBs further. The protoplast extraction in Dendrobium required modification due to the accumulation of mucilage and crystallization inside the flower tissue.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Supatida Abdullakasim

Role: Thesis Advisor

Name: Cattleya Chutteang

Role: Thesis Co-Advisor

Name: Shogo Matsumoto

Role: Thesis Co-Advisor

Created: 2022

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2565/thanachok-tati-all.pdf

CallNumber: QK495.O64.T43

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Agricultural Sciences

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	thanachok-tati-all.pdf	5.8 MB

ใช้เวลา

0.037198 วินาที