Innovative development of novel synbiotic products using Thai pigmented rice and honey

Name: Swe, Zin Myo.

LCSH: Kasetsart University -- Dissertations. Ph.D. (Bioproducts Science) 2022.

Classification :.LCCS: QR82.L3

LCSH: Kasetsart University. -- Department of Science -- Dissertations.

LCSH: Lactobacillus

LCSH: Stingless bees.

LCSH: Rice products.

LCSH: Probiotics.

LCSH: Functional foods.

Abstract: In this study, Limosilactobacillus reuteri KUKPS6103, Lacticaseibacillus rhamnosus KUKPS6007, Lacticaseibacillus paracasei KUKPS6201, Lactobacillus acidophilus KUKPS6107, Ligilactobacillus salivarius KUKPS6202, Bacillus coagulans KPSTF02 and Saccharomyces boulardii KUKPS6005 are applied both in the production of synbiotic products based on pigmented rice cultivars and stingless bee honey, and in the production of lyophilized-probiotic product using the complex medium (wall materials). The results of antibacterial activity for the synbiotic production showed that all of the probiotic bacteria had the ability to inhibit six strains of food borne pathogen (Aeromonas hydrophila KPS-01, Bacillus cereus KPS-01, Escherichia coli KPS01, Proteus vulgaris KPS-01, Staphylococcus aureus KPS-01 and Salmonella typhimurium KPS-01). All probiotic strains were performed to determine the antioxidant activity using ABTS*+ radical scavenging assay, DPPH assay, cholesterol removal, biofilm formation and GABA production. Among the three probiotic bacteria for the synbiotic products; L. reuteri KUKPS6103 had the high antioxidant activity showing with 1.564 ± 0.060 mg Trolox mL-1 probiotic extract analyzed with ABTS assay. Similarly, cholesterol removal was also analyzed for the three probiotic bacteria and mix-cultured. The result was observed that the growth probiotic strain in the honey was notified by the turbidity of the probiotic-diluted honey. On the other hand, the stingless bee honey had the enhancing capacity in the prebiotic studies and the main carbohydrate sources were sucrose, glucose and fructose. A synbiotic product was produced enriching with the Thai-pigmented rice grains (Riceberry, Tubtim Chumphae and Sangyod) and the stingless bee honey. Likewise, the most biofilm formation occured in L. reuteri KUKPS6103 and the least in B. coagulans KPSTF02. The multi strain probiotic for lyophilized probiotic product had the highest cholesterol removal. All the probiotic strains had GABA production for lyophilized-probiotic product. For the microbiological safety, it was indicated that the amounts of contaminant were acceptable. In addition, the study successfully developed a synbiotic product, which gives much benefits to human health. And, the findings provided a new type of wall material that is safer and more effective and, can be extensively applied in relevant food applications.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Saran Promsai

Role: Thesis Advisor

Name: kanjana Pangjit

Role: Thesis Co-Advisor

Created: 2022

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2565/zin-myo-all.pdf

CallNumber: QR82.L3.S94

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Bioproducts Science

Grantor: Kasetsart University

©copyrights Kasetsart University

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