Development of multi-strain synbiotic product using pigmented rice and rice bran oil

Name: Thapakorn Chumphon.

LCSH: Kasetsart University -- Dissertations. Ph.D. (Bioproducts Science) 2022.

Classification :.LCCS: TP684.R5

LCSH: Kasetsart University. -- Department of Science -- Dissertations.

LCSH: Rice oil.

LCSH: Probiotics.

LCSH: Functional foods.

LCSH: Pigments.

Abstract: PUBLICATION I This study found that the isolated probiotics Lactobacillus paracasei KUKPS6201, L. acidophilus KUKPS6107, L. reuteri KUKPS6103, L. rhamnosus KUKPS6007, L. salivarius KUKPS6202, Bacillus coagulans KPSTF02 and Saccharomyces boulardii KUKPS6005 had high potential for probiotic properties. All strains had antibacterial activity and high antioxidant activity of 1.654 ± 0.017 mg Trolox mL−1 probiotic extract. The selected strains could survive in a simulated gastrointestinal tract under anaerobic conditions and showed no haemolytic activity. Furthermore, the probiotic strains were strongly autoaggregated and also showed co-aggregated ability with pathogenic bacteria. The probiotic microorganisms demonstrated high ability to adhere to Thai-pigmented rice grains. The results of analysis of these probiotics showed that Riceberry rice bran oil was an excellent prebiotic. A synbiotic product containing Thai pigmented rice grains (cultivar Riceberry, Luem Pua and Black Jasmine) and rice bran oil was produced. After 8 weeks of storage, the viability of the probiotics in terms of multistrains was 7.36 ± 0.04 log CFU g –1 (85.78% survival rate). Microbiological safety testing indicated that the amounts of contaminants were acceptable. This study provided the first scientific report on the feasibility of applying Thai-pigment rice, rice bran oil and mixed-culture probiotics as a novel functional synbiotic product. PUBLICATION II Background: Thailand is one of the most bio-diversifid countries in Southeast Asia, especially regarding plants, animals and microbes that impact directly on human and animal life. Methods: Of the 201 bacterial isolates that were screened from Thai rice and bees in Ratchaburi and Chiang Mai provinces, Thailand, most were classifid in the genus Bacillus. Result: From the morphological test and 16S ribosomal DNA sequence study, three strains, Bacillus flexus KRptI_S2, Microbacterium paraoxydans C13HN2 and Paenibacillus hunanensis KRrb_T2, could produce amylase and protease enzyme and nonhemolytic activity at higher temperature. The highest enzymatic activity of protease was produced by P. hunanensis KRrb_T2 (378.9967±1.335 U.mL-1 ) at 36 h.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Saran Promsai

Role: Thesis Advisor

Name: Kanjana Pangjit

Role: Thesis Co-Advisor

Created: 2022

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2565/thapakorn-chum-all.pdf

CallNumber: TP684.R5.T43

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Bioproducts Science

Grantor: Kasetsart University

©copyrights Kasetsart University

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