Clinical evaluation of hypertrophic cardiomyopathy in maine coon cats using loop-mediated isothermal amplification and proteomics

Name: Pratch Sukumolanan

LCSH: Kasetsart University -- Dissertations. Ph.D. (Veterinary Clinical Studies) 2023.

Classification :.LCCS: SF986.C38

LCSH: Kasetsart University. -- Faculty of Veterinary Medicine -- Dissertations.

LCSH: Cats -- Diseases.

LCSH: Genetic disorders in animals -- Diagnosis.

LCSH: Myocardium -- Diseases.

Abstract: Hypertrophic cardiomyopathy (HCM) is a common primary cardiovascular disease in cats. The pathophysiology of feline HCM is still controversial. It has been reported that familial feline HCM is associated with myosin-binding protein C-3 (MYBPC3) gene mutation. However, the information on risk factors, pathophysiology, and candidate novel biomarkers in HCM cats with MYBPC3 gene mutation is limited, and the screening test for MYBPC3-A31P gene mutation in cats is still required. Therefore, this thesis study aimed to assess clinical data and risk factors for HCM associated with MYBPC3 gene mutation. Moreover, additional purposes of this study were an invention of a loop-mediated isothermal amplification (LAMP) with a lateral flow dipstick test (LFD) for MYBPC3-A31P diagnosis (A31P-LAMP-LFD) and serum peptidomics investigation in cats with sarcomeric gene mutation. Sixty-three Maine Coon cats were enrolled. Complete cardiovascular examination and echocardiography were determined. Genotyping of MYBPC3 gene mutation was completed with polymerase chain reaction (PCR) and Sanger sequencing. A31P-LAMP-LFD was meticulously developed. The optimal protocol for A31P-LAMP was examined. Peptidomics was analyzed with matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and liquid chromatography with tandem mass spectrometry (LCMS/MS). The results revealed that MYBPC3 in Maine Coon cats was 36.51 % in Thailand. Moreover, we found that the risk associated with HCM included being male, overweight (exceeding 6 kg), increasing age (exceeding 37 months), and prolonged isovolumic relaxation time (IVRT) (exceeding 46 ms). For A31PLAMP-LFD, we accomplished distinguishing normal cats and MYBPC3-A31P mutation cats with high sensitivity, high specificity, cost-effectiveness, and less time-consuming. The optimized time and temperature of A31P-LAMP were 60 minutes at 64°C. Besides, peptidomics analysis discovered that the proteins among control cats, Maine Coon cats with and without MYBPC3 mutation, were absolutely separated. The peptide mass fingerprints (PMFs) represented the specific peaks in each group. The levels of FOXO1, AGAP2, and CYP3A132 were elevated in Maine Coon with MYBPC3 mutation. However, the knowledge of feline HCM with early detection, disease mechanism, and therapeutic management is further warranted.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Soontaree Petchdee

Role: Thesis Advisor

Name: Janjira Phavaphutanon

Role: Thesis Co-Advisor

Created: 2023

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2566/pratch-suku-all.pdf

CallNumber: SF986.C38.P73

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Veterinary Clinical Studies

Grantor: Kasetsart University

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