Application of wisteria floribunda agglutinin in Cholangiocarcinoma

Name: Winunya Phuyathip.

LCSH: Kasetsart University -- Theses. M.P.H. 2023.

Classification :.LCCS: RC280.B48

LCSH: Kasetsart University. -- Department of Public Health Administration -- Theses.

LCSH: Bile ducts -- Cancer -- Treatment.

LCSH: Cancer -- Treatment.

LCSH: Lectins.

LCSH: Wisteria.

LCSH: Agglutinins.

Abstract: Cholangiocarcinoma is considered a malignant type of cancer because it is difficult to diagnose in the early stages and there is no specific CCA indicator yet. Therefore, majority of patients are found in a late stages. In this study, we investigate the potential of Wisteria floribunda agglutinin (WFA) for the detection of WFAbinding glycans in Cholangiocarcinoma (CCA) tissues by identifying the roles of WFA-binding glycans in CCA cell lines. WFA was used to determine the expression of α, β-GalNAc glycan expression in KKU-213A, KKU-213B, KKU-055, and MMNK-1 CCA cell lines. The data showed that α and β-GalNAc were higher expressed in KKU-213A and KKU-213B while lower expressed in KKU-KKU-055, and MMNK-1. In addition, for the roles of WFA-binding glycan, we investigate the signaling pathway using Western Blot, according to the cell proliferation from the MTT assay, KKU-213A and KKU-213B cell lines were treated with 0, 0.001, 0.01, 0.1, 1 µg/ml respectively of WFA for 72 hr. The results showed that the cell viability of KKU-213A and KKU-213B cell lines were significantly increased after treatment with WFA at 0, 0.001, 0.01, 0.1, 1 µg/ml respectively. Therefore, this data confirms that WFA-binding glycan can activate cell growth via P-AKT and P-ERK signaling pathway and this study suggest the activation would be involved in the metastasis of CCA.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Sriwipa Chuangchaiya

Role: Thesis Advisor

Created: 2023

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2566/winunya-phuy-all.pdf

CallNumber: RC280.B48.W56

eng

DegreeName: Master of Public Health

Level: Master's Degree

Descipline: Public Health

Grantor: Kasetsart University

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