Biotechnological exploration of Rambutan (Nephelium lappaceum L) peel extraction of ellagitannins and cellulose fiber as bio-based materials for application in food, cosmetic, pharmaceutical and biomedical industries

Name: Torgbo, Selorm.

LCSH: Kasetsart University -- Dissertations. Ph.D. (Biotechnology) 2022.

Classification :.LCCS: QK495.S19

LCSH: Kasetsart University. -- Department of Biotechnology -- Dissertations.

LCSH: Rambutan -- Utilization.

LCSH: Cellulose.

LCSH: Plant Extracts.

LCSH: Tannins.

LCSH: Plant bioactive compounds.

Abstract: The idea of circular economy creates the avenue to recycle and process agro-industrial waste as a bioresource into secondary raw materials. This study explored rambutan peel (RP) as a sustainable alternative lignocellulosic biomass for cellulose and bioactive compounds production. Three extraction techniques; ohmic heating (OH), Soxhlet and accelerated solvent extractions (ASE) were accessed in parallel for ellagitannin (E) extraction and also as a pretreatment method for cellulose production. In all, the ASE extraction technique with water and water-acetone (20:80) in sequence gave the highest yield of E (51.46%). Mineral elements such as Ca, Fe, K, Mg, Mn and Na were identified in the extracts. Ellagitannin rich extract was obtained via partitioning of Soxhlet extract, which showed high total phenolic content (713.4 ± 21.9 mg/GAE/g) and antioxidant activities similar to that of vitamin C. Geraniin is the most dominant compound and highest contributor of antioxidant activity (61.34%). The antioxidant activity is equivalent to that of the commercial geraniin (1.56 ± 0.11 Trolox equivalent g/g). The extract was effective against Staphylococcus aureus and epidermidis and Cutibacterium acnes, with low toxicity on fibroblast L929 cells. Bread fortified with OH extract at 15 µg/mL fortification level showed better phenolic content, antioxidant activity, good texture properties and antifungal activity. For fibers, different bleaching conditions; sodium chlorite, sodium hydroxide with hydrogen peroxide and peracetic acid (PAA) were study for cellulose production. The synergetic enzymatic treatment before sodium chlorite bleaching reduced chemical input by 28% with high crystallinity index of fibers (65.8%). The study showed RP contains high amount of lignin (> 30%) followed by α-cellulose of 28.3 ± 0.6% and hemicellulose (>19%). However, using greener approach with 5% PAA bleaching is more economical with 70% reduction of bleaching time and chemical input by 38%. Carbohydrates such as arabinose rhamnose, xylose, 4OMeGlcUA, galactose, GalUA and glucose were present in an appreciable quantity in extractives and fibers. An aqueous-based cellulose gel prepared from fibers showed shear thinning behavior. Cellulose hydrogel was also prepared by functionalizing cellulose fibers (F) with gelatin (G) at different proportions (GF 1:1, 0.5:1 and 0:1) and immobilized with E. The E immobilization significantly increased the rheological and compression properties of the hydrogel. Rambutan peel as a bioresource is a promising sustainable alternative to lignocellulosic biomass for application in pharmaceutical, cosmetic, food and biomedical industries.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Prakit Sukyai

Role: Thesis Advisor

Name: Udomlak Sukatta

Role: Thesis Co-Advisor

Created: 2022

Modified: 2025-06-23

Issued: 2025-06-23

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2565/selorm-tor-all.pdf

CallNumber: QK495.S19.T67

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Biotechnology

Grantor: Kasetsart University

©copyrights Kasetsart University

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