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Warisara Raksachue. Preparation of levan from B. subtilis under different cultivation conditions. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2024.
Preparation of levan from B. subtilis under different cultivation conditions
Name: Pornpun Aramsangtienchai
Organization :
Burapha University. Faculty of Science
Email :
Pornpun.ar@go.buu.ac.th
Name: Warisara Raksachue
Organization :
Burapha University. Faculty of Science
LCSH:
Fructans
LCSH:
Bacillus subtilis
LCSH:
Polysaccharides
LCSH:
Fermentation
Abstract:
Levan is a fructan biopolymer consisting of fructose molecules linked via beta-2, 6 glycosidic bond.
It is typically synthesized through the catalysis levansucrase, secreted from certain microorganisms.
Interestingly, variations in the molecular weight (MW) of levan have been associated with different levels of
biological activities, including anti-bacterial and antitumor effects. However, the preparation of different MW
of microbial levan is still challenging. Herein, we investigated the MW of levan produced from Bacillus
subtilis under different cultivation conditions. Our findings revealed that the MW of levan from B. subtilis
cultured in minimal medium was 1.8 x 104 Da. It was higher than the MW of levan from cells in a complete
medium, which was 9 x 103 Da. Additionally, shaking speed (Round Per Minute, RPM) also impacted the
levan MW. Specifically, the levan MW of 1.7 x 104 Da and 9.8 x 103 Da were detected at the shaking speed
of 100 and 180 RPM, respectively. The results of this study thus provided cultivation conditions for preparing
levan with different MWs from B. subtilis. Also, it will facilitate further investigation of biological activities
associated with levan of varying MW
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2024
Modified:
2024-12-11
Issued:
2024-12-11
บทความ/Article
application/pdf
BibliograpyCitation :
In Kasetsart University. Faculty of Science. The Pure and Applied Chemistry International Conference 2024 (PACCON 2024) (pp.1055-1058). Bangkok : Kasetsart University, 2024
eng
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Pornpun Aramsangtienchai
| Title | Contributor | Type |
|---|---|---|
| Preparation of levan from B. subtilis under different cultivation conditions
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pornpun Aramsangtienchai;Warisara Raksachue | บทความ/Article |
Warisara Raksachue
| Title | Contributor | Type |
|---|---|---|
| Preparation of levan from B. subtilis under different cultivation conditions
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pornpun Aramsangtienchai;Warisara Raksachue | บทความ/Article |