Aptamer-based lateral flow biosensor for the detection of cadmium

Name: Vorawan Yurayart

Organization : Chulalongkorn University. Faculty of Science

Name: Sirowan Ruantip

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Yakoh, Abdulhadee

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Sudkate Chaiyo

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Email : Sudkate.c@chula.ac.th

keyword: Lateral flow immunoassay

LCSH: Biosensors

; Aptamers

LCSH: Heavy metals -- Environmental aspects

LCSH: Environmental monitoring

Abstract: Currently, chemical test kits are popular for detecting cadmium (Cd2+) contamination in food and the environment. However, this device still has many steps of operation. Here, an aptamer-based lateral flow biosensor was developed for the rapid detection of Cd2+. This sensor combines a flow test strip with an aptasensor not only to increase the specificity for Cd2+ detection but also to reduce the detection steps of the device. In the operating principle of this strip test, the Cd-aptamer probe was mixed with the sample before being applied to the sample pad. In the absence of Cd2+, the Cd-aptamer probe was free, and then this solution continuously flowed into a conjugate pad coated with an AuNPs conjugated probe. This free Cd-aptamer probe was hybridized with the AuNPs conjugated probe so that the test line did not appear as a color band. The cadmium detection was interpreted as negative. In the case of positive, Cd2+ ions are specifically bound to the Cd-aptamer probe. Next, this solution continued to flow to the conjugate pad, which could not bind to the AuNPs conjugated probe. For this reason, the test line appears as a colored band from the binding of the AuNPs conjugated probe, and the aptamer sequences match the test line. This test strip has been shown to detect as low as 9 ppb of Cd2+. As an additional suggestion, this test strip could potentially be applied to detect Cd2+ in tap water samples, demonstrating its ease of use, rapidity, and field readiness

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2024

Modified: 2024-11-25

Issued: 2024-11-25

บทความ/Article

application/pdf

BibliograpyCitation : In Kasetsart University. Faculty of Science. The Pure and Applied Chemistry International Conference 2024 (PACCON 2024) (pp.86-90). Bangkok : Kasetsart University, 2024

eng

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