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Yupaporn Sameenoi. Paper-based test strip for analysis of organophosphate pesticides. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2024.
Paper-based test strip for analysis of organophosphate pesticides
Name: Wanchalearm Manchan
Organization :
Burapha University. Faculty of Science
Name: Yupaporn Sameenoi
Organization :
Burapha University. Faculty of Science
Email :
yupaporn@buu.ac.th
keyword:
Organophosphates
LCSH:
Pesticides
-- Analysis
LCSH:
Analytical chemistry
LCSH:
Food
-- Safety measures
Abstract:
A paper-based test strip for analysis of organophosphate pesticide using enzyme inhibition assay has
been developed. The strip composed of two ends for control and sample test zones. Both zones were
immobilized with the acetylcholinesterase enzyme (AChE) and Ellman's Reagent (DTNB). In the absence of
pesticides, the enzyme AChE catalyzed the formation of thiocholine (TC) that reacts with DTNB to produce
a yellow product (TNB-). After incubation with organophosphate pesticide, the activity of AChE was inhibited
and produced less TC, and hence, reduced yellow product. Color intensity was analyzed by scanning the
paper-based test strip and measuring the intensity using ImageJ software. This developed assay is able to
analyze organophosphate pesticides with the detection limits of organophosphate pesticides as low as 0.025
μg/mL of methyl-paraoxon. The developed method has been successfully applied to detect methyl-paraoxon
in spiked cabbage and kale obtaining recovery values about 95% and 96%, respectively. Therefore, the
developed method is promising for analysis of organophosphate pesticides in real samples
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2024
Modified:
2024-11-25
Issued:
2024-11-25
บทความ/Article
application/pdf
BibliograpyCitation :
In Kasetsart University. Faculty of Science. The Pure and Applied Chemistry International Conference 2024 (PACCON 2024) (pp.30-35). Bangkok : Kasetsart University, 2024
eng
©copyrights King Mongkut's University of Technology North Bangkok
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ใช้เวลา
0.019708 วินาที
Wanchalearm Manchan
| Title | Contributor | Type |
|---|---|---|
| Paper-based test strip for analysis of organophosphate pesticides
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wanchalearm Manchan;Yupaporn Sameenoi | บทความ/Article |
Yupaporn Sameenoi
| Title | Contributor | Type |
|---|---|---|
| Paper-based test strip for analysis of organophosphate pesticides
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wanchalearm Manchan;Yupaporn Sameenoi | บทความ/Article | |
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มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wannisa Sroisarika;Yupaporn Sameenoi | บทความ/Article | |
| Paper-based devices for water hardness analysis
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Alicha Marut;Yupaporn Sameenoi | บทความ/Article | |
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