Production and certification of certified reference material of andrographolide in Andrographis Paniculata powder by a network of competent laboratories approach

Name: Ponhatai Kankaew

Organization : National Institute of Metrology. Chemical Metrology and Biometry department

Email : pornhatai@nimt.or.th

Name: Kittiya Shearman

Organization : National Institute of Metrology. Chemical Metrology and Biometry department

Name: Kanjana Hongthong

Organization : National Institute of Metrology. Chemical Metrology and Biometry department

Name: Cheerapa Boonyakong

Organization : National Institute of Metrology. Chemical Metrology and Biometry department

Name: Charun Yafa

Organization : National Institute of Metrology. Chemical Metrology and Biometry department

keyword: Andrographis paniculata

LCSH: Medicinal plants -- Standards

; Andrographolide

LCSH: Reference sources

LCSH: Botanical chemistry

Abstract: Andrographis paniculata is in Thailand's official list of essential medicines (Herbal medicine list) from the Ministry of Public Health for the management of respiratory and gastrointestinal disorders, including COVID-19 treatment. It was recommended that the proper amount of active pharmaceutical ingredient (Andrographolide) for COVID-19 treatment is 180 mg/day. Accurate measurement of the amount of active ingredient in herbal medicine is, therefore, important, not only for the effectiveness of treatment, but also for minimizing potential for drug overdose. There were no certified reference materials available to allow laboratories to measure their analytical method performance. A matrix-matched Certified Reference Material (CRM), TRM-F-5011, was prepared at the National Institute of Metrology, Thailand (NIMT), from freeze dried, ground, Green Chiretta (Andrographis Paniculata) leaves. Homogeneity and stability of the material were rigorously assessed. Characterization of the reference material was carried out by a network of competence laboratories. The interlaboratory results were statistically analyzed, and median was calculated as assigned value of CRM. Measurement uncertainty of the certified value was evaluated from the betweenunit inhomogeneity, long-term storage stability of the material at -20 °C, and laboratory biases from characterization by a network of competence laboratories. The results showed that TRM-F-5011 contained Andrographolide at (18.7 ± 1.3) mg/g.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2024

Modified: 2024-11-25

Issued: 2024-11-25

บทความ/Article

application/pdf

BibliograpyCitation : In Kasetsart University. Faculty of Science. The Pure and Applied Chemistry International Conference 2024 (PACCON 2024) (pp.1-6). Bangkok : Kasetsart University, 2024

eng

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