Taxonomy and phylogeny of endophytic fungi associated with Amomum tsao-ko in Yunnan, China, and screening for antagonistic activities

Name: Peixuan Yu

Address: 333 Moo1, Thasud, Muang, Chiang Rai 57100

Organization : Mae Fah Luang University

Email : library@mfu.ac.th

keyword: Multi-locus Phylogeny

MeSH: Phylogeny

Classification :.DDC: e-thesis

; New Species

MeSH: Morphology

MeSH: Antagonistic fungi -- 311234

MeSH: Biology -- Classification -- 59923

MeSH: Endophytic fungi -- 61662

LCSH: Phylogeny

LCSH: Morphology

LCSH: Antagonistic fungi -- 311234

LCSH: Biology -- Classification -- 59923

LCSH: Endophytic fungi -- 61662

Abstract: The dried fruits of Amomum tsao-ko are used as both medicinal herbs and food additives, and the compounds isolated from the fruits of the plant have great clinical value. The aim of this study was to investigate the diversity of culturable endophytic fungi in Amomum tsao-ko in Yunnan using four different low-strength media. A total of 319 fungal strains were isolated, and preliminary ITS analyses showed that they belonged to 3 phyla, 7 classes, 14 orders, 36 families and 54 genera. The dominant genera are Acremonium, Aspergillus, Mucor, Penicillium, and Sarocladium. Fusarium graminearum is a pathogenic bacterium that causes Amomum tsao-ko wilt, which severely reduces the yield and quality of Amomum tsao-ko. The aim of the present study was to screen and identify antagonistic strains against Fusarium graminearum and to assess their biosynthetic potential. The antagonistic strains were screened by dual-culture plate antagonism assay and identified in combination with internal transcribed spacer (ITS) gene sequencing. Detection of polyketide synthase (PKS-I and PKS-II) and non-ribosomal peptide synthase (NRPS) genes in antagonistic strains by specific amplification of chromosomal DNA. Among the 127 endophytic fungal strains used for antagonistic screening, 89 strains showed antagonism, and 18 strains with high antagonism (inhibition rate > 50%), of which the two strains with the highest inhibition rates were Trichoderma harzianum and Penicillium scabrosum, with inhibition rates as high as 85.93%, but containing only the NRPS gene. The highest percentage of the 18 highly antagonistic strains belonged to the genus Penicillium. Based on these results, Penicillium sp. surfaces as a dominant endophyte in Amomum tsao-ko, which has great potential for exploitation in the control of pathogens. An endophytic species of Exophiala was isolated from the fruits of Amomum tsao-ko during the investigation of the fungal diversity associated with Amomum tsao-ko in Honghe Prefecture, Yunnan Province, China. The sequences of ITS, LSU, and SSU were compared to those of closely related species. Exophiala hongheensis sp. nov is introduced based on phylogenies and morphological characters. The new species is phylogenetically related to E. lecanii-corni, E. lavatrina and E. mali, however, it can be distinguished from those by its smaller conidia and conidiogenous cells on the lateral side of the mycelium. Detailed descriptions, illustrations and phylogenies of E. hongheensis are provided herein.

Mae Fah Luang University. Learning Resources and Educational Media Centre

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Pattana Kakumyan

Created: 2024

Modified: 2024-10-29

Issued: 2024-10-29

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: e-thesis

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biological Sciences

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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