Guiding Sclerotia Formation in Sclerotinia sclerotiorum Through Tolerating Impinging Stress

Name: Chang HD,

Address: 333 Moo1, Thasud, Muang, Chiang Rai 57100

Organization : Center of Excellence in Fungal Research, Mae Fah Luang University

Email : library@mfu.ac.th

Name: Tsai H,

Name: Chang YK,

Name: Liu MA,

keyword: Fungal physiology

ThaSH: Fungi

; Sclerotial initial site

ThaSH: Fungi -- Physiology

; Cytochemical localization

ThaSH: Metabolism

; Secondary metabolism

; Cushion aggregation

; Sclerotinia sclerotium

Abstract: The sclerotium of Sclerotinia sclerotiorum produces its primary inoculum ascospore through the sexual cycle, enhancing pathogenicity via meiotic genetic variation. The formation of the sclerotium plays a pivotal role in developing genetic variation for adaptation. This study specifically examines the transfer of energy-conserving agents to the resting body. The approach involves establishing a 2-D position on the medium's surface, elucidating the transition from a floating state to a defined position through the manipulation of allowable stress. Direct monitoring under the abiotic impinge stress model indicates that strengthened sites experiencing maximum stress modulate the process. As gametes were indistinguishable, the differentiation of vegetative hyphae was observed to be swollen, parallel-aligned, and eventually wound and aggregated. Notably, development exhibits tolerance to lower temperatures. As suggested, the experiment delves into cell differentiation from vegetative to storage bodies, with biochemical mechanisms playing a crucial role. To glean insights into structures based on morphogenesis time series, the model provides a platform for the direct examination of metabolic activity in the initial stages. Apart from structural morphology, cytochemical staining immediately after tissue fixation indicates the dominance of bio-energetic activity, provided by reducing sugars and succinate dehydrogenase (SUDH) activity, with increases in the differential apex and parallel alliances for cellular development. The distribution of glucose-6PDH activity fluctuates over time, intermittently aligning with SUDH through all stages. Polyphenol oxidase activity is sparse in developing cells and increases after the cushion aggregation (CA) stage. The effects of metabolic inhibitors highlight the role of respiration and RNA synthesis inhibition at effective doses, underscoring their significance in sclerotia development. Control amino acid (AA) doses reveal enhanced profiles, including aromatic, branched, and long-chain structures stimulated in the fusion of parallel mycelium clumps. Finally, the nuclei of ascospores in the sexual stage possess two nuclei, a characteristic feature of S. sclerotiorum.

Mae Fah Luang University. Learning Resources and Educational Media Center

Address: CHIANG RAI

Email: library@mfu.ac.th

Modified: 2024-06-06

Issued: 2024-06-06

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BibliograpyCitation : Asian Journal of Mycology (AJOM). Vol.7, No.1 (January - June 2024): p.55-77

Asian Journal of Mycology (AJOM), Vol.7 No.1 (2024): p55-77

eng

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