Establishment of method for Wolbachia transinfection into the leafhopper, vector of sugarcane white leaf disease

Name: Kamonrat Suwanchaisri

Organization : Thammasat University. Faculty of Science and Technology

Name: Jureemart Wangkeeree

Organization : Thammasat University. Faculty of Science and Technology

Email : jureemart@yahoo.com

LCSH: Wolbachia

LCSH: Leafhoppers

LCSH: Sugarcane -- Diseases and pests

Abstract: Microinjection offers transfection the biological materials that are injected into target insects; it’s used to study interaction of insects and injected foreign substances. Wolbachia is an intracellular symbiont that can manipulates insect host biology in numerous ways. Wolbachia has attracted attention as a potential novel biological control agent against the increase of vector-borne human and insect plant diseases. However, many important insect pests are uninfected or are infected with strains of Wolbachia that do not have a critical role and are suitable to be used in the control. The desirable strains of Wolbachia can be transferred into new hosts (transinfection), which create infection status in important insect pests. The leafhopper Matsumuratettix hiroglyphicus (Matsumura), insect vectors of the phytoplasma that cause white leaf disease in sugarcane and was undetectable of Wolbachia infection. The microinjection technique has been no developed in this insect vector; therefore, we have established appropriate procedure microinjection for delivery solution substances and study the successful of Wolbachia transinfection into the M. hiroglyphicus leafhopper. The results found that PBS solution had no significant difference on survival rate in injection sites between ventral and lateral site of the abdomen (45.83 and 51.85%, respectively) when compared with control solution. In addition, PBS solution also resulted the normal adult longevity (averaged 37.83 days for male and 37.94 days for female) and reproductive traits in the M. hiroglyphicus leafhopper. The obtained results was used as procedure microinjection for delivery Wolbachia into this insect. After the injection, the injected M. hiroglyphicus showed 64.52% positivity (20 out of 31) and 41.67% positivity (5 out of 12) for Wolbachia infection at 0 and 7 day post-injection, respectively. The density of Wolbachia in injected adult M. hiroglyphicus was average as 25.54 x 103 copies of wsp gene. This result would provide highlights the importance of further studies on the use of Wolbachia as a biological control agent for the leafhopper vector.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2023

Modified: 2024-05-01

Issued: 2024-05-01

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BibliograpyCitation : In Rangsit University. The 8th RSU International Research Conference on Sciences and Technology 2023 (RSUCON 2023) (pp.411-419). Pathum Thani : Rangsit University

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