Micro-computed tomography analysis of bone regeneration using MRNA encoding bone morphogenetic protein-2 with implant placement in rat femur

Name: Nopparada Lawtrakulngam

Organization : Chulalongkorn University. Faculty of Dentistry

Name: Philaiporn Vivatbutsiri

Organization : Chulalongkorn University. Faculty of Dentistry

Name: Noppadol Sa-Ard-Iam

Organization : Chulalongkorn University. Faculty of Dentistry

Name: Jaijam Suwanwela

Organization : Chulalongkorn University. Faculty of Dentistry

Email : jaijam1220@gmail.com

keyword: Bone morphogenetic protein 2

LCSH: Bone regeneration

LCSH: Messenger RNA

LCSH: Femur

LCSH: Microcomputed tomography

Abstract: Bone morphogenetic protein-2 (BMP-2) has a potential for enhancing bone regeneration and osseointegration around implant materials. However, the drawback of BMP-2 protein delivery is instability. Therefore, high doses of BMP-2 need to be used clinically, which results in higher costs and more side effects. Although gene-based therapy, in the form of mRNA BMP-2, can overcome these limitations. Recent clinical studies on mRNA BMP-2 regarding bone regeneration around implant materials are lacking. This study aims to investigate the ability of N1-methylpseudouridine-modified mRNA encoding bone morphogenetic protein-2 (m1Ψ-BMP-2 mRNA) to enhance bone regeneration after implant placement in rat femur defects. Twelve implant titanium wires were placed into 6 Sprague-Dawley (SD) rats. The femur defects were randomly filled with 15 μg m1Ψ-BMP-2 mRNA (n = 4), 4 μg recombinant human bone morphogenetic protein-2 (rhBMP-2) (n = 4), or Dulbecco's phosphate-buffered saline (dPBS) (n = 4). The animals were sacrificed 6 weeks after implantation. The percentages of bone volume fraction (%BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and bone-to-implant contact (BIC) were evaluated using micro-computed tomography imaging (micro-CT). One-way ANOVA was used to analyze the data between the three groups to compare BMP-2 effectiveness. All statistical analysis was performed at a significant level of 5% (p-value < 0.05). Bone volume and trabecular micro-architecture parameters were significantly greater in the m1Ψ-BMP-2 mRNA group than the dPBS group. In conclusion, these results reveal a positive effect of m1Ψ-BMP-2 mRNA that results in increased bone formation at the peri-implant area compared to rhBMP-2 and dPBS

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2023

Modified: 2024-05-01

Issued: 2024-05-01

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BibliograpyCitation : In Rangsit University. The 8th RSU International Research Conference on Sciences and Technology 2023 (RSUCON 2023) (pp.276-285). Pathum Thani : Rangsit University

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