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Nisarat Ruangsawasd. Antibiofilm activities of the self-prepared silver solution against streptococcus mutans biofilm : in vitro. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2023.
Antibiofilm activities of the self-prepared silver solution against streptococcus mutans biofilm : in vitro
Name: Suthara Asanahsark
Organization :
Mahidol University. Faculty of Dentistry
Name: Praphasri Rirattanapong
Organization :
Mahidol University. Faculty of Dentistry
Email :
praphasri.rir@mahidol.ac.th
Name: Nisarat Ruangsawasd
Organization :
Mahidol University. Faculty of Dentistry
keyword:
Biofilm inhibition
LCSH:
Silver
Abstract:
Dental caries is a common chronic disease which is one of many factors, caused by Streptococcus mutans biofilm. This study aimed to determine the ability of the self-prepared silver solution toward S. mutans biofilm formation and eradication. The study design contained 2 parts; biofilm inhibition test along with susceptibility test of the self-prepared silver solution. The experiment design for biofilm inhibition test was microplate assay with crystal violet. The testing solutions were two-fold serial dilution from the self-prepared silver solution 300 μg/mL and 1.5% TSB as positive control. The biofilm susceptibility test of the self-prepared silver solution was done with 2 different methods; CFU assay and Live/Dead assay. The CFU assay was done with testing solutions at 300, 150, 50,15, and 5 μg/mL of the self-prepared silver solution and 1% NaOCl as positive control. Live/Dead assay was done with the same testing solutions and the biofilms were observed under Confocal Laser Scanning Microscope (CLSM) and percentage of dead bacteria was calculated. All experiments were repeated three times, Data were analyzed with one-way ANOVA and Tukeys multiple comparison.
The results showed that the lowest concentration of the self-prepared silver solution that could inhibit S. mutans biofilm formation was 2.34 μg/mL (p-value < 0.0001) and the lowest concentration that could eradicate S. mutans cell in biofilm form was 300 μg/mL (p-value < 0.05). The percentage of dead bacteria under CLSM of 300 μg/mL of the self-prepared silver solution was 51 ± 28.18. This study concluded that the minimum biofilm inhibition concentration and eradication concentration of the self-prepared silver solution is 2.34 and 300 μg/mL, respectively
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2023
Modified:
2024-04-29
Issued:
2024-04-29
บทความ/Article
application/pdf
BibliograpyCitation :
In Rangsit University. The 8th RSU International Research Conference on Sciences and Technology 2023 (RSUCON 2023) (pp.205-212). Pathum Thani : Rangsit University
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.032448 วินาที
Suthara Asanahsark
| Title | Contributor | Type |
|---|---|---|
| Antibiofilm activities of the self-prepared silver solution against streptococcus mutans biofilm : in vitro
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Suthara Asanahsark;Praphasri Rirattanapong;Nisarat Ruangsawasd | บทความ/Article |
Praphasri Rirattanapong
| Title | Contributor | Type |
|---|---|---|
| Antibiofilm activities of the self-prepared silver solution against streptococcus mutans biofilm : in vitro
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Suthara Asanahsark;Praphasri Rirattanapong;Nisarat Ruangsawasd | บทความ/Article |
Nisarat Ruangsawasd
| Title | Contributor | Type |
|---|---|---|
| Antibiofilm activities of the self-prepared silver solution against streptococcus mutans biofilm : in vitro
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Suthara Asanahsark;Praphasri Rirattanapong;Nisarat Ruangsawasd | บทความ/Article |