Evaluation of antibacterial and antibiofilm activities of polyherbal formulation extract in combination with antibiotics against Staphylococcus aureus and Streptococcus pyogenes skin disease-causing bacteria

Name: Rohana Dolee

keyword: Herbal Formulation Extract

MeSH: Antibacterial agents -- 15735

Classification :.DDC: e-Theses

; Antibacterial Activity

MeSH: Staphylococcus aureus -- 174498

MeSH: Streptococcus pyogenes -- 308368

MeSH: Antibiotics

MeSH: Herbs -- Extracts -- 308369

LCSH: Antibacterial agents -- 15735

LCSH: Staphylococcus aureus -- 174498

LCSH: Streptococcus pyogenes -- 308368

LCSH: Antibiotics

LCSH: Herbs -- Extracts -- 308369

Abstract: Skin infections caused by bacteria, particularly Staphylococcus aureus and Streptococcus pyogenes, remain a significant health concern. This study investigates the antibacterial and antibiofilm activities of an herbal formulation extract (HFE) prepared with the Anacardium occidentale, Zingiber montanum, and Aloe vera against these pathogens, both HFE alone and in combination with selected antibiotics. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of HFE were determined against reference and clinical strains by the microdilution technique and time-kill study to assess the time-independence. Synergistic interactions between HFE and different antibiotics were assessed by the checkerboard method. Furthermore, the crystal violet assay and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) reduction assay in combination were used to observe antibiofilm effects. HFE reveals potent antibacterial effects, particularly against S. pyogenes, with MIC in the reference and isolated strains of 64 and 8 µg/ml, respectively. Time-kill studies demonstrated the rapid and dose-dependent bactericidal activity of HFE against the tested strains. Antibiotic susceptibility testing highlighted high resistance, emphasizing the urgency for alternative treatment strategies. Combining HFE with antibiotics revealed synergistic effects in 2.90% of tested combinations against S. aureus but none against S. pyogenes. Notably, the combination of HFE with amoxicillin and ampicillin exhibited the most pronounced synergistic effects, reducing MIC values and potentially mitigating antibiotic resistance. Moreover, the antibiofilm activity of HFE was assessed, showing significant inhibition of biofilm formation and eradication of biofilms, particularly in combination with selected antibiotics. These findings suggest a dual action of HFE, targeting both bacterial growth and biofilm, which is crucial for addressing persistent infections associated with biofilm production. In conclusion, the present study underscores the potent antibacterial and anti-biofilm activities of HFE, particularly in combination with specific antibiotics against S. aureus. The observed synergistic effects suggest a promising strategy for enhancing antibacterial effectiveness and addressing biofilm-related challenges. Further research is warranted to elucidate the underlying mechanisms and evaluate the safety and efficacy of HFE in clinical settings.

Mae Fah Luang University. Learning Resources and Educational Media Center

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Jongkon Saising

Role: Advisor

Created: 2023

Modified: 2024-03-05

Issued: 2024-03-05

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: e-Theses

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Health and Biomedical Analytics

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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