Efficacy of beneficial microorganism for plant growth stimulation and plant immunity to diseases in rice

ประสิทธิภาพของจุลินทรีย์ที่มีประโยชน์ต่อการกระตุ้นการเจริญเติบโตของพืชและการสร้างภูมิคุ้มกันโรคในข้าว

Name: Rungrat Vareeket

ThaSH: Rice -- Weed control

ThaSH: Rice -- Growth

ThaSH: Microbial pesticides

ThaSH: Plant growth promoting substances

Abstract: The research findings found Curvularia lunata causing leaf spot of rice var. RD41 and PL2 and Drechslera oryzae (brown leaf spot of rice var. RD47 and RD57). c. lunata and D. oryzae were proved by pathogenicity. The antagonistic bacteria were found as Rhodospirillum centenum strains SM41, SM61, SM72 and SM92. R. centenum strains SM41, SM61, SM72 and SM92 found to be gram¬negative, motile cells, gram negative, expressed reddish color and shiny convex colony. It was negative for gelatin activity test. R. centenum strains SM41 and SM61 expressed to good fermentation activities of glucose, sucrose and lactose and isolates SM72 and SM92 found to be non-fermented activity R. centenum strains SM41, SM61, SM72 and SM92 were also proved by molecular phylogenic through analysis of 16S rRNA gene sequence which closely related to Rhodospirillum centenum SW in Genbank No CP00613.2. The other antagonistic bacteria, Serratia marcescens strain LB 01 showed gram-negative, rod shape, motility, gelatin positive, red culture, and smooth appearance. Dietzia cercidiphylli strain LB02 showed orange in color, smooth, gram-positive, rod, non-motile, and gelatin negative. Further research findings, R. centenum SM41, SM61, SM72, SM92. ร. marcescens (LB01) and D. cercidiphylli (LB02) were shown to produce amylase, protease and lipase which possible proved to be control mechanism. R. centenum SM41 treated to rice var. RD41 promoted seed germination of rice var. PL2 and followed by R. centenum strains SM92, SM61 and SM72. R. centenum strain SM92 applied to rice var. RD41 gave a good plant strand of rice and R. centenum strains SM41, SM61, SM72 and SM92 stimulated seed germination on rice var. RD41 and var. PL2. The antagonistic action of R. centenum strains SM41 and SM61 decreased leaf spot disease and stimulated plant growth of rice. Serratia marcescens (LB01) and Dietzia cercidiphylli (LB02) were found to be antagonize c. lunatra causing leaf spot of rice var. RD41 and promoted plant growth of rice var RD41. The antagonistic fungus, Neosartorya hiratsukae (EU06) found to be inhibited D. oryzae (brown leaf spot of rice var. RD47). Chaetinium brasiliense (CB01) proved to be inhibited the tested D. oryzae. Crude extracts of Ch. brasiliense (CB01) inhibited the tested!), oryzae.Crude ethyl acetate metabolite of A. hiratsukae EU06 proved to be inhibited Drechslera oryzae causing brown leaf spot of rice var. RD47 the ED₅₀ of 12.18 ppm, and followed by crude hexane and methanol metabolite which the ED₅₀ of 29.16 and 43.90 ppm, respectively. Crude ethyl acetate extract of Ch. brasiliense CB01 showed highest inhibition to the tested D. oryzae which the ED₅₀ of 9.64 ppm, and followed by crude hexane and methanol metabolites which the ED₅₀ were 14.35 and 23.35 ppm, respectively. Research investigation on nanotechnology for plant immunity was found that nano-NHM from N. hiratsukae EU06 inhibited D. oryzae (brown leaf spot of rice var. RD47). Nano-NHM, nano-NHE and nano-NHH inhibited sporulation with the ED50 values of 3.93, 3.97 and 4.46 ppm, respectively. Morover, nano-CBH, nano-CBE, and nano-CBM from Ch. brasiliense CB01 inhibited sporulation of D. oryzae with ED₅₀ of 0.83, 1.70, and 2.59 ppm, respectively. Nano-CBH, nano-CBE, nano-CBM treated to the inoculated rice var. RD4 7 with D. oryzae which proved to produce Oryzalexin c on the thin layer chromalography where the retention factor (Rf) is 0.61

King Mongkut's Institute of Technology Ladkrabang. Central Library

Address: BANGKOK

Email: Lifelong@kmitl.ac.th

Name: Kasem Soytong

Role: Thesis Advisor

Name: Supattra Poeaim

Role: Thesis Co-Advisor

Created: 2023

Modified: 2567-01-24

Issued: 2024-01-24

วิทยานิพนธ์/Thesis

application/pdf

eng

Level: Doctoral Degree

Descipline: Agriculture

Grantor: King Mongkut's Institute of Technology Ladkrabang

DegreeName: Doctor of philosophy

©copyrights King Mongkut's Institute of Technology Ladkrabang

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