Product development of plant essential oil Nanoemulsions for controlling phyllotreta sinuata stephens, plutella xylostella linnaeus and spodoptera litura fabricius

การพัฒนาผลิตภัณฑ์น้ำมันหอมระเหยนาโนอิมัลชันจากพืชในการควบคุมด้วงหมัดผัก, หนอนใยผักและหนอนกระทู้ผัก

Name: Hataichanok Passara

ThaSH: Essences and essential oils

ThaSH: Plant extracts

Abstract: Abstract:Cruciferous vegetables were considered as the king of vegetables and super food. The main cause of the damage to the production of cruciferous vegetables for export was insect pests affecting the quality and quantity of crop production. Especially, flea beetle (PhyUotreto sinuoto Stephens), diamondback moth (Ptutetta xylostella Linnaeus), and cutworm (Spodoptera litura Fabricius) in which may cause 100% of economic yield loss. Therefore, in case of plant production agriculturists widely used chemical insecticides to control the insect pests. Chemical insecticide and its residue remaining in environment could pose risks to non-target organism. Therefore, this study aimed to develop eco-friendly and less toxic insecticides from plant essentials oil in form of nanoemulsions for controlling flea beetle, diamondback moth and cutworm. The initial screening test of plant essential oils (EOs) obtained from 12 selected plants, namely cardamon, eucalyptus, holy basil, sweet basil, betel vine, cinnamon, lemongrass, star anise, clove, long pepper, black pepper, and turmeric against those insect pests was performed by using leaf dipping bioassay method. Organic Chinese cabbage leaves of 3 cm in diameter were dipped into EOs and dried at room temperature for 15 minutes. After that 10 adult stages of flea beetles and second stage of instar larvae of diamondback moth and cutworm were put in the testing box. The mortality rates of these adults and larvae were observed at 24 hours. The results showed that at 1% concentration of star anise and clove essential oil gave the mortality rates of flea beetle with 100%. Whereas, 1% concentration of star anise and turmeric essential oil presented the mortality rates of diamondback moth and cutworm with 100%. Therefore, star anise, turmeric, and clove were subjected to develop as essential oil nanoemulsions. Essential oil nanoemulsions (nEOs) from star anise, turmeric, clove and their main chemical compounds were tested against flea beetle, diamondback moth, and cutworm to evaluate their mortality, antifeedant, growth inhibition, and repellent activities. Essential oil nanoemulsion (nEO) was prepared by mixing the selected plant essential oils with various surfactants or co-surfactants by aqueous titration method Abstract: After that particle sizes and zeta potential of nanoemulsions were measured by Nanoplus Zeta/Nano Particle Analyzer. As a result, the nEO particle sizes were smaller than 100 nm. The nEOs of star anise, turmeric and clove were tested against those insects by using leaf dipping method with the observation at 24 hours. The results showed that nEO of star anise at concentrations of 0.35%, 0.20%, 0.20%, and 0.03% had the highest level of toxicity against the flea beetle with choice and no-choice antifeedant activities, as well as repellent activities at 100%, 100%, 100%, and 81.67%, respectively. The results also demonstrated that nEOs of star anise at 0.35%, 0.20%, 0.20%, 0.30% and 0.20% had the highest level of toxicity against diamondback moth with mortality, in choice and no-choice antifeedant activities, and growth inhibitions in pupae and adults at 100%. The nEOs of this plant at concentrations of 0.35%, 0.15%, 0.20%, 0.30% and 0.20% also gave the highest level of toxicity against cutworm with mortality, in choice and no-choice antifeedant activities, and growth inhibition in pupae and adults at 100%. The nEOs of star anise and deltamethrin (chemical insecticide) were aimed to test with adult stage of flea beetles and second stage of instar larvae of diamondback moth and cutworm by the same method. The results showed 100% mortality of flea beetles, diamondback moth, and cutworm at 0.35% concentration of star anise nanoemulsions where, deltamethrin at recommendation rate gave only 96.70% (flea beetle), 98.30% (diamondback moth) and 95.00% (cutworm) mortality. เท addition, when star anise nEO and deltamethrin were applied by using direct spraying method against the flea beetle, diamondback moth, and cutworm in greenhouse and field conditions, it revealed that at 0.35% concentration of star anise nEO could control by 100% and 85.45%, respectively. When deltamethrin at recommendation rate performed only 65.38% (flea beetle in greenhouse conditions), 46.91% (diamondback moth in greenhouse conditions), 58.55% (cutworm in greenhouse conditions) and 49.39% (field conditions) controls. Therefore, star anise nEO seem to be used as a highly-effective and environmentally friendly pesticide to control flea beetle, diamondback moth, and cutworm

Address: BANGKOK

Email: Lifelong@kmitl.ac.th

Name: คำรณวิทย์ ทิพย์มณี

Role: อาจารย์ที่ปรึกษาวิทยานิพนธ์

Created: 2023

Modified: 2024-01-25

Issued: 2567-01-24

วิทยานิพนธ์/Thesis

application/pdf

eng

Descipline: Agriculture

Grantor: King Mongkut's Institute of Technology Ladkrabang

DegreeName: Doctor of philosophy

Level: Doctoral Degree

©copyrights King Mongkut's Institute of Technology Ladkrabang

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