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Nguyen, Ha Vu Hong.. Optimization of enzyme-assisted extraction of bioactive peptides from whiteleg shrimp (Litopenaeus vannamei) head waste using box-behnken design. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2024.
Optimization of enzyme-assisted extraction of bioactive peptides from whiteleg shrimp (Litopenaeus vannamei) head waste using box-behnken design
Name: Nguyen, Duong Canh.
Organization :
International University. Biotechnology School
Name: Nguyen, Vy Le.
Organization :
International University. Biotechnology School
Name: Nguyen, Ha Vu Hong.
Organization :
International University. Biotechnology School
Email :
nvhha@hcmiu@hcmiu.edu.vn
keyword:
Bioactive peptides.
LCSH:
Whiteleg shrimp.
LCSH:
Animal waste.
LCSH:
Amylases
-- Inhibitors.
Abstract:
Whiteleg shrimp (Litopeneaus vannamei) processing lines discard huge quantities of head wastes, which are the protein-rich source. Enzymatic hydrolysis has been studied for decades to salvage discarded whiteleg shrimp head waste to produce high-added value products applicable to animal industry. Alcalase 2.5 L, which is widely applied on marine materials, was used to hydrolyze whiteleg shrimp head waste protein and then purified by cold absolute ethanol. Hydrolysis conditions including enzyme/substrate (E/S) ratio, incubation temperature and duration were preliminarily screened (E/S ratio 0-2.5 percent, temperature 50-70 degree celsius, duration 30-90 min) before conducting optimization using Box-Behnken design. After optimization, collected protein hydrolysate reached an experimental yield of 61.64 percent on dry basis under the optimal conditions as treated by Alcalase 2.5 L at 0.9 percent E/S ratio and incubated at 62 degree celsius for 65 min. Protein hydrolysate performed bioactivity including DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity (3.88 ± 0.10 mg Trolox equivalents g dry matter-1), reducing power (19.20 ± 0.52 mg Trolox equivalents g dry matter-1) and alpha-amylase inhibitory activity (10.74 ± 0.65 percent inhibition at 50 mg mL-1)."
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2024
Modified:
2567-01-16
Issued:
2024-01-16
บทความ/Article
application/pdf
BibliograpyCitation :
APPLIED SCIENCE AND ENGINEERING PROGRESS. vol. 17, no. 1 (Jan.-Mar. 2024), p. 1-13.
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.04848 วินาที
Nguyen, Duong Canh.
| Title | Contributor | Type |
|---|---|---|
| Optimization of enzyme-assisted extraction of bioactive peptides from whiteleg shrimp (Litopenaeus vannamei) head waste using box-behnken design
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Nguyen, Duong Canh.;Nguyen, Vy Le.;Nguyen, Ha Vu Hong. | บทความ/Article |
Nguyen, Vy Le.
| Title | Contributor | Type |
|---|---|---|
| Optimization of enzyme-assisted extraction of bioactive peptides from whiteleg shrimp (Litopenaeus vannamei) head waste using box-behnken design
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Nguyen, Duong Canh.;Nguyen, Vy Le.;Nguyen, Ha Vu Hong. | บทความ/Article |
Nguyen, Ha Vu Hong.
| Title | Contributor | Type |
|---|---|---|
| Optimization of enzyme-assisted extraction of bioactive peptides from whiteleg shrimp (Litopenaeus vannamei) head waste using box-behnken design
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Nguyen, Duong Canh.;Nguyen, Vy Le.;Nguyen, Ha Vu Hong. | บทความ/Article |