Abstract:
Mushrooms are foods and herbs that have been consumed for a long time. This is due to the mushroom's numerous health benefits. To obtain pharmacological benefits, it is necessary to consume adequate quantities of a broad variety of mushrooms. Therefore, the objective of this study was to develop a formulation and preparation process for a food supplement from mixed mushroom polysaccharide (Bhutan oyster mushroom, white oyster mushroom, gray oyster mushroom, and Lingzhi mushroom) in the form of instant beverage powder. The prepared product is a fine, white powder that contains a uniform distribution of brown powdered mushroom extract. The optimal formulation (E3) had mostly particle sizes in the range of 300400 micrometers with a uniform size distribution, and the flow property is fair. The percentage of moisture content is 4.38, which is within the acceptable range (1-5%). The formulation has good water solubility; the solubility in warm water (60 °C) is better than that in normal-temperature water (25 °C), as well as having a lower viscosity. According to the results of stability testing run under accelerated conditions for one month at a temperature of 40°C ± 2°C and a relative humidity of 75% ± RH 5%, the beverage powder packaged in aluminum foil pouches has not changed in weight or physical properties compared to the product prior to the stability test. Pea protein powder was added to provide the mixed mushroom beverage with complete nutrients, resulting in the best formulation containing mixed mushroom polysaccharide extract 25 mg, MCT powder 15%, sucrose 5%, stevia 1.5%, flavor 1%, pea powder 40%, and maltodextrin 37.5% w/w. The final product has complete nutrients, good physical properties, and a pleasant taste, ensuring that it is suitable for continued development in industry and commercialization.