Bio-based solvent for lipid extraction from oleaginous yeast Rhodotorula glutinis and intensification extractability by physical-assisted pretreatment

Name: Tullawat Ketvaraporn

Organization : Chiang Mai University. Graduate School

Name: Pensiri Sriburi

Organization : Chiang Mai University. Faculty of Science

Name: Jidapha Tinoi

Organization : Chiang Mai University. Faculty of Science

Email : jidapha.t@cmu.ac.th

keyword: Rhodotorula glutinis

ThaSH: Organic solvents

ThaSH: Extraction (Chemistry)

ThaSH: Fatty acids -- Analysis

Abstract: This research aims to find an alternative lipid extraction from oleaginous yeast, Rhodotorula glutinis. The bio-based solvent extractions with/without physical-assisted methods were investigated and compared to the conventional extraction (chloroform: methanol, 2:1, v/v) in term of the relative lipid extractability. The results presented that the bio-based solvents, acetone, ethyl acetate: ethanol, and ethyl acetate: isopropanol gave the high relative extractability of lipid as 54.36+1.87 percent, 46.00+2.88 percent, and 44.80+7.00 percent of total lipids, respectively, whereas the conventional method showed the relative lipid extractability of 38.98+1.90 percent. Moreover, the physical pretreatments; microwave radiation, ultrasonic, and bead milling were applied for cell pretreatment prior to solvent extraction. The results proved that the physical pretreatment could enhance the lipid extractability from R. glutinis cells. The physical-assisted bio-based solvent revealed the highest relative lipid extractability was 84.68+5.70 Percent of total lipid by microwave-assisted ethanol extraction, followed by microwave-assisted acetone extraction (76.76+1.87 percent) and ultrasonic-assisted acetone (67.50+3.15 percent). For the intensified lipid extractability, microwave-assisted ethanol indicated the highest increase of lipid extractability to 49.73 percent, compared to the microwave-assisted acetone was 22.40 percent increased. The results demonstrated that the alternative bio-based solvents have a potential for lipid extraction from R. glutinis cells and could substitute the commonly used solvent. Furthermore, the microwave-assisted pretreatment prior to bio-based solvent extraction markedly showed the proper method to intensify the lipid extractability significantly.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2020

Modified: 2023-12-15

Issued: 2023-12-15

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BibliograpyCitation : In the Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn in association, Thammasat University Faculty of Science and Technology. The Pure and Applied Chemistry International Conference 2020 (PACCON 2020) (CB48-53). Bangkok : Thammasat University

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